EARLY, POSTNATAL EXPERIENCE ALTERS HYPOTHALAMIC CORTICOTROPIN-RELEASING FACTOR (CRF) MESSENGER-RNA, MEDIAN-EMINENCE CRF CONTENT AND STRESS-INDUCED RELEASE IN ADULT-RATS
EARLY, POSTNATAL EXPERIENCE ALTERS HYPOTHALAMIC CORTICOTROPIN-RELEASING FACTOR (CRF) MESSENGER-RNA, MEDIAN-EMINENCE CRF CONTENT AND STRESS-INDUCED RELEASE IN ADULT-RATS
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DOI：
10.1016/0169-328x(93)90189-v
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发表时间：
1993-05-01
期刊：
影响因子：
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通讯作者：
MEANEY, MJ
中科院分区：
文献类型：
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作者：
PLOTSKY, PM;MEANEY, MJ
每天将2-14日龄的幼鼠暴露于处理（与母体和饲养笼分离15分钟）、母体分离（MS; 180分钟的相当分离）或完全不受干扰（未处理; NH）。作为成年人，MS大鼠下丘脑促肾上腺皮质激素释放因子（CRF）的mRNA水平增加与NH大鼠相比，而CRF的mRNA水平在H大鼠显着低于MS或NH动物。基础条件下丘脑CRF含量遵循完全相同的模式。一个20分钟的限制应力期间产生显着的CRF消耗在所有组中，虽然消耗的百分比是显着较低的H动物相比，无论是MS或NH动物。约束压力产生了显着较高的增加，血浆皮质酮在MS和NH动物比H动物。这些数据反映了早期环境因素在调节下丘脑CRF系统的发展和下丘脑-垂体-肾上腺轴对应激的反应性方面的重要性。
Rat pups 2-14 days of age were exposed daily to handling (15 min of separation from mother and home cage), maternal separation (MS; 180 min of comparable separation), or were left entirely undisturbed (non-handled; NH). As adults, MS rats showed increased hypothalamic corticotropin-releasing factor (CRF) mRNA levels compared with NH rats, while CRF mRNA levels in H rats were significantly lower than either MS or NH animals. Hypothalamic CRF content under basal conditions followed exactly the same pattern. A 20-min period of restraint stress produced significant CRF depletion in all groups, although the percentage of depletion was significantly lower in H animals compared with either MS or NH animals. Restraint stress produced significantly higher increases in plasma corticosterone in MS and NH animals than in H animals. These data reflect the importance of early environmental factors in regulating the development of the hypothalamic CRF system and the responsiveness of the hypothalamic-pituitary-adrenal axis to stress.