Importance of a 5′ stem-loop for longevity of papA mRNA in Escherichia coli
Importance of a 5′ stem-loop for longevity of papA mRNA in Escherichia coli
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DOI：
10.1128/jb.181.11.3587-3590.1999
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发表时间：
1999-06-01
影响因子：
3.2
通讯作者：
Belasco, JG
中科院分区：
文献类型：
--
作者：
Bricker, AL;Belasco, JG
大肠杆菌尿路致病菌株的主要菌毛亚基 (PapA) 的高水平表达部分是由于编码该蛋白的 mRNA 的异常长寿命所致。在这里，我们报告说 papA mRNA 的寿命在很大程度上源自其 5' 非翻译区提供的保护。这个 papA RNA 片段可以延长与其融合的短寿命 mRNA 的寿命。体内烷基化研究表明，在自然环境中，papA 信息以茎环结构开始。该茎环对于 papA 5' 非翻译区的稳定作用非常重要，其去除导致 papA mRNA 衰减显着加速就证明了这一点。
High-level expression of the major pilus subunit (PapA) of uropathogenic strains of Escherichia coli results in part from the unusually long lifetime of the mRNA that encodes this protein. Here we report that the longevity of papA mRNA derives in large measure from the protection afforded by its 5' untranslated region. This papA RNA segment can prolong the lifetime of an otherwise short-lived mRNA to which it is fused. In vivo alkylation studies indicate that, in its natural milieu, the papA message begins with a stem-loop structure. This stem-loop is important for the stabilizing effect of the papA 5' untranslated region, as evidenced by the significant acceleration in papA mRNA decay that results from its removal.