13C-NMR analysis of alanine metabolism by isolated perfused livers from C3HeB/FeJ mice infected with African trypanosomes.
13C-NMR analysis of alanine metabolism by isolated perfused livers from C3HeB/FeJ mice infected with African trypanosomes.
复制标题
对感染非洲锥虫的 C3HeB/FeJ 小鼠的分离灌注肝脏的丙氨酸代谢进行 13C-NMR 分析。
DOI：
10.1016/0305-0491(88)90308-2
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Scott,AI
中科院分区：
文献类型：
--
作者：
Hall,JE;Mackenzie,NE;Mansfield,JM;McCloskey,DE;Scott,AI
1. Isolated perfused livers from mice infected with Trypanosoma brucei rhodesiense formed substantially more [3-13C]-lactate from [3-13C]-alanine than livers from uninfected mice. Quantities formed by infected livers increased as infection progressed. 2. Infected livers produced more 13C-labeled glutamate and glutamine, with label scrambled between C-2 and C-3. Scrambling also produced [2, 3-13C]-aspartate,[2-13C]-alanine and [2-13C]-lactate. Delayed appearance of label in C-4 of glutamate/glutamine in infected livers reflects significant endogenous stores of unlabeled acetyl CoA. 3. Although differences do exist in catabolism of [3-13C]-alanine by perfused livers from infected and control mice, trypanosomiasis does not cause permanent breakdown or blockage of hepatic alanine metabolism.
