CLEAVAGE OF PYRIMIDINE DIMERS IN SPECIFIC DNA-SEQUENCES BY A PYRIMIDINE DIMER DNA-GLYCOSYLASE OF M-LUTEUS
CLEAVAGE OF PYRIMIDINE DIMERS IN SPECIFIC DNA-SEQUENCES BY A PYRIMIDINE DIMER DNA-GLYCOSYLASE OF M-LUTEUS
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DOI：
10.1038/285634a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
GROSSMAN, L
中科院分区：
文献类型：
--
作者：
HASELTINE, WA;GORDON, LK;GROSSMAN, L
对紫外线辐射响应的嘧啶二聚体的形成是由潜在二聚体和两个侧翼核苷酸的胸腺嘧啶含量决定的。可以从黄分支杆菌中纯化一种酶活性，该酶活性可以在不断裂磷酸二酯键的情况下，裂解二聚体的5‘’-嘧啶和相应的糖之间的N-糖基键。黄色分枝杆菌酶在二聚体位置的链断裂需要两种活性，一种是嘧啶二聚体DNA糖基酶，另一种是脱嘧啶/无嘌呤核酸内切酶。
Pyrimidine dimer formation in response to UV radiation is governed by the thymine content of the potential dimer and the 2 flanking nucleotides. An enzymatic activity can be purified from M. luteus that cleaves the N-glycosyl bond between the 5'' pyrimidine of a dimer and the corresponding sugar without rupture of a phosphodiester bond. Strand scission at a dimer site by the M. luteus enzyme requires 2 activities, a pyrimidine dimer DNA-glycosylase and an apyrimidinic/apurinic endonuclease.