The Role of Tape Measure Protein in Nucleocytoplasmic Large DNA Virus Capsid Assembly.
The Role of Tape Measure Protein in Nucleocytoplasmic Large DNA Virus Capsid Assembly.
复制标题
卷尺蛋白在核胞质大 DNA 病毒衣壳组装中的作用。
DOI：
10.1089/vim.2020.0038
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发表时间：
2021
期刊：
影响因子：
2.2
通讯作者：
Xiao,Chuan
中科院分区：
文献类型：
--
作者：
Xian,Yuejiao;Avila,Ricardo;Pant,Anil;Yang,Zhilong;Xiao,Chuan
核质大DNA病毒（Nucleocytoplasmic large DNA viruses，NCLDVs）是一类能感染从动物到原生生物等多种宿主的大型病毒。根据基因组序列分析，这些病毒在NCLDV中被分组在一起。它们共享一组病毒体形态发生和复制所必需的基因。大多数NCLDV通常具有较大的物理尺寸，而它们的形态在不同的家族中变化，例如二十面体，砖形或椭圆形，这引起了对其形态发生的可能调节因素的问题。二十面体NCLDV的衣壳由称为衣壳体的小结构单元组装而成，衣壳体是主要衣壳蛋白的三聚体形式。注意，未成熟痘病毒的衣壳是球形的，即使它们是由与那些二十面体NCLDV共享高度结构保守性的衣壳组装而成的。最近发表的NCLDV、绿草履虫小球藻病毒1和非洲猪瘟病毒的高分辨率结构描述了位于衣壳下方的小衣壳蛋白的密集网络。在这些次要的蛋白质中，有一种延伸的卷尺蛋白质（TmP），它从一个二十面体的五重顶点跨越到另一个。在这项研究中，我们专注于TmP在二十面体NCLDV衣壳组装中发挥的关键作用，回答了先前提出的螺旋机制中提出的一个问题。有趣的是，在TmPs上的基本局部比对搜索显示痘病毒中没有显著的命中，这可能是区分痘病毒和二十面体NCLDV在其形态发生中的因素。
Nucleocytoplasmic large DNA viruses (NCLDVs) are a group of large viruses that infect a wide range of hosts, from animals to protists. These viruses are grouped together in NCLDV based on genomic sequence analyses. They share a set of essential genes for virion morphogenesis and replication. Most NCLDVs generally have large physical sizes while their morphologies vary in different families, such as icosahedral, brick, or oval shape, raising the question of the possible regulatory factor on their morphogenesis. The capsids of icosahedral NCLDVs are assembled from small building blocks, named capsomers, which are the trimeric form of the major capsid proteins. Note that the capsids of immature poxvirus are spherical even though they are assembled from capsomers that share high structural conservation with those icosahedral NCLDVs. The recently published high resolution structure of NCLDVs,Paramecium bursariaChlorella virus 1 and African swine fever virus, described the intensive network of minor capsid proteins that are located underneath the capsomers. Among these minor proteins is the elongated tape measure protein (TmP) that spans from one icosahedral fivefold vertex to another. In this study, we focused on the critical roles that TmP plays in the assembly of icosahedral NCLDV capsids, answering a question raised in a previously proposed spiral mechanism. Interestingly, basic local alignment search on the TmPs showed no significant hits in poxviruses, which might be the factor that differentiates poxviruses and icosahedral NCLDVs in their morphogenesis.