Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction
Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction
复制标题
DOI：
10.1161/circulationaha.105.537480
复制
发表时间：
2005-09-06
期刊：
影响因子：
37.8
通讯作者：
Bulte, JWM
中科院分区：
文献类型：
--
作者：
Kraitchman, DL;Tatsumi, M;Bulte, JWM
背景-最近的动物研究结果表明，干细胞可能能够定位于心肌损伤部位，以帮助组织再生。方法和结果-利用单光子发射CT(SPECT)/CT联合扫描仪的高灵敏度，动态测定了放射性示踪剂和MR造影剂共标记的同种异体间充质干细胞(MSCs)在急性心肌梗死中的体内运输。静脉注射后，观察到标记的MSCs在注射后24-48小时内从最初定位于肺到非靶器官如肝、肾和脾的重新分布。心肌梗死后24小时SPECT/CT图像上可见梗死区MSCs的局限性和弥漫性摄取，并持续到注射后7天，并经组织放射性计数证实。相反，MRI无法显示MSCs的心脏定位，部分原因是MRI的敏感性较低。结论：无创性放射性核素成像非常适合于动态追踪间充质干细胞的生物分布和向靶器官和非靶器官的运输。
Background - Recent results from animal studies suggest that stem cells may be able to home to sites of myocardial injury to assist in tissue regeneration. However, the histological interpretation of postmortem tissue, on which many of these studies are based, has recently been widely debated.Methods and Results - With the use of the high sensitivity of a combined single-photon emission CT (SPECT)/CT scanner, the in vivo trafficking of allogeneic mesenchymal stem cells (MSCs) colabeled with a radiotracer and MR contrast agent to acute myocardial infarction was dynamically determined. Redistribution of the labeled MSCs after intravenous injection from initial localization in the lungs to nontarget organs such as the liver, kidney, and spleen was observed within 24 to 48 hours after injection. Focal and diffuse uptake of MSCs in the infarcted myocardium was already visible in SPECT/CT images in the first 24 hours after injection and persisted until 7 days after injection and was validated by tissue counts of radioactivity. In contrast, MRI was unable to demonstrate targeted cardiac localization of MSCs in part because of the lower sensitivity of MRI.Conclusions - Noninvasive radionuclide imaging is well suited to dynamically track the biodistribution and trafficking of mesenchymal stem cells to both target and nontarget organs.