Imaging Mass Spectrometry with Silver Nanoparticles Reveals the Distribution of Fatty Acids in Mouse Retinal Sections
Imaging Mass Spectrometry with Silver Nanoparticles Reveals the Distribution of Fatty Acids in Mouse Retinal Sections
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DOI：
10.1016/j.jasms.2010.04.005
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发表时间：
2010-08-01
影响因子：
3.2
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Hayasaka, Takahiro;Goto-Inoue, Naoko;Setou, Mitsutoshi
利用纳米粒子辅助激光解吸电离成像质谱（nano-PALDI-IMS）中的银纳米粒子（AgNPs），以负离子模式建立了一种新的小鼠肝脏和视网膜样品中脂肪酸的可视化方法。到目前为止，IMS分析集中在主要的细胞成分，如细胞膜磷脂和细胞骨架肽。用烷基羧酸盐和烷基胺修饰的AgNP用于纳米PALDI-IMS以鉴定小鼠肝脏切片中的脂肪酸，例如硬脂酸、油酸、亚油酸、花生四烯酸和二十碳五烯酸以及棕榈酸;使用2，5-二羟基苯甲酸（DHB）作为基质不能检测到这些脂肪酸。使用nano-PALDI-IMS测定棕榈酸的检测限为50 pmol。将nano-PALDI-IMS方法成功应用于小鼠肝脏切片脂肪酸离子图像的重建。我们通过分析标准脂质样品验证了小鼠肝组织切片中脂肪酸的检测，结果表明，当用激光照射时，脂肪酸来自游离脂肪酸和脂质中的游离脂肪酸。此外，我们将所提出的方法应用于识别小鼠视网膜组织切片中的脂肪酸，这使我们能够了解视网膜不同层中脂肪酸的六带分布。我们相信，目前在纳米PALDI-IMS中使用AgNPs的方法可能会导致一种新的策略，通过脂肪酸的分布来分析基本的生物学机制和几种疾病。（J Am Soc Mass Spectrom 2010，21，1446-1454）（C）2010年美国质谱学会
A new approach to the visualization of fatty acids in mouse liver and retinal samples has been developed using silver nanoparticles (AgNPs) in nanoparticle-assisted laser desorption/ionization imaging mass spectrometry (nano-PALDI-IMS) in negative ion mode. So far, IMS analysis has concentrated on main cell components, such as cell membrane phospholipids and cytoskeletal peptides. AgNPs modified with alkylcarboxylate and alkylamine were used for nano-PALDI-IMS to identify fatty acids, such as stearic, oleic, linoleic, arachidonic, and eicosapentaenoic acids, as well as palmitic acid, in mouse liver sections; these fatty acids are not detected using 2,5-dihydroxybenzoic acid (DHB) as a matrix. The limit of detection for the determination of palmitic acid was 50 pmol using nano-PALDI-IMS. The nano-PALDI-IMS method is successfully applied to the reconstruction of the ion images of fatty acids in mouse liver sections. We verified the detection of fatty acids in liver tissue sections of mice by analyzing standard lipid samples, which showed that fatty acids were from free fatty acids and dissociated fatty acids from lipids when irradiated with a laser. Additionally, we applied the proposed method to the identification of fatty acids in mouse retinal tissue sections, which enabled us to learn the six-zonal distribution of fatty acids in different layers of the retina. We believe that the current approach using AgNPs in nano-PALDI-IMS could lead to a new strategy to analyze basic biological mechanisms and several diseases through the distribution of fatty acids. (J Am Soc Mass Spectrom 2010, 21, 1446-1454) (C) 2010 American Society for Mass Spectrometry