CDX2 expression induced by leukocytapheresis might be associated with mucosal healing in ulcerative colitis.
CDX2 expression induced by leukocytapheresis might be associated with mucosal healing in ulcerative colitis.
复制标题
白细胞去除术诱导的 CDX2 表达可能与溃疡性结肠炎的粘膜愈合有关。
DOI：
10.1111/jgh.13645
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Watanabe M
中科院分区：
文献类型：
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作者：
Tsuchiya K;Hayashi R;Fukushima K;Hibiya S;Horita N;Negi M;Itoh E;Akashi T;Eishi Y;Motoya S;Takeuchi Y;Kunisaki R;Fukunaga K;Nakamura S;Yoshimura N;Takazoe M;Iizuka B;Suzuki Y;Nagahori M;Watanabe M
背景与目的溃疡性结肠炎（Ulcerative colitis，UC）是一种顽固性、反复发作的慢性结肠炎性疾病。虽然最近UC治疗的目标是靶向粘膜愈合，但粘膜愈合的分子机制仍不清楚。在这项研究中，我们的目的是阐明相关的细胞分离治疗过程中肠上皮细胞的增殖和分化的分子动力学在短duration.MethodsEndoscopy进行了26例UC患者在多中心医院，活检标本收集前和两周内从直肠白细胞分离（LCAP）后。结果LCAP后尾型同源框2（caudal type homeobox 2，CDX 2）和hes家族bHLH转录因子1（hes family bHLH transcription factor 1，HES 1）的表达明显增加。LCAP后内镜改善的患者在LCAP前显示CDX2的表达。此外，杯状细胞的数量显着增加后LCAP。LCAP后无内镜改善的患者在LCAP前未显示CDX2的表达。然而，CDX2的表达显着增加后LCAP.ConclusionThis研究表明，cytapheresis可能诱导CDX2的表达，而不影响细胞增殖，从而导致粘膜愈合与杯状细胞恢复。
Background and AimUlcerative colitis (UC) is a chronic inflammatory disease of the colon with an intractable, recurrent course. Although the goal of UC therapy has recently been to target mucosal healing, the molecular mechanism of mucosal healing remains unknown. In this study, we aimed to elucidate the molecular dynamics related to the proliferation and differentiation of intestinal epithelial cells during cytapheresis therapy in a short duration.MethodsEndoscopy was performed in 26 patients with UC in multicentre hospitals, and biopsy specimens were collected from the rectum before and within two weeks after leukocytapheresis (LCAP). The expression of representative proteins in intestinal epithelial cells and pathological findings was compared before and after LCAP.ResultsThe expression of caudal type homeobox 2 (CDX2) and a hes family bHLH transcription factor 1(HES1) markedly increased after LCAP. Patients with endoscopic improvement after LCAP showed the expression of CDX2 before LCAP. Moreover, the number of goblet cells significantly increased after LCAP. Patients without endoscopic improvement after LCAP did not show the expression of CDX2 before LCAP. However, the expression of CDX2 markedly increased after LCAP.ConclusionThis study suggests that cytapheresis might induce CDX2 expression without affecting the cell proliferation, thus resulting in mucosal healing with goblet cell restoration.