The 5' regulatory region from the Drosophila pseudoobscura hsp82 gene results in a high level of reporter gene expression in Lucilia cuprina embryos
The 5' regulatory region from the Drosophila pseudoobscura hsp82 gene results in a high level of reporter gene expression in Lucilia cuprina embryos
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DOI：
10.1016/0378-1119(96)00149-7
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发表时间：
1996-10-10
期刊：
影响因子：
3.5
通讯作者：
Atkinson, PW
中科院分区：
文献类型：
--
作者：
Coates, CJ;Howells, AJ;Atkinson, PW
我们之前已经检测了两个黑腹果蝇启动子在澳大利亚绵羊苍蝇Lucilia cuprina胚胎中实现报告基因表达的效率。HSP70热休克启动子和actin5C启动子都导致报告基因在这些胚胎中的低水平表达。本研究检测了拟黑僵菌hsp82启动子(Phsp82)对大肠杆菌氯霉素乙酰转移酶编码基因(CAT)表达的影响。我们报道了在美洲斑潜蝇胚胎中的CAT活性水平与在黑腹果蝇中获得的相同结构的CAT活性水平相当，表明phsp82在这种非果蝇昆虫中有效地发挥作用。结果表明，phsp82可能被用于其他非果蝇类昆虫，在这些昆虫中，含有HSP70或actin5C启动子的构建体表达水平较低。
We have previously examined the efficiency of two Drosophila melanogaster promoters to enable reporter gene expression in embryos of the Australian sheep blowfly, Lucilia cuprina. Both the hsp70 heat-shock promoter and the actin5C promoter resulted in low levels of expression of a reporter gene in these embryos. In this study, the D. pseudoobscura hsp82 promoter (phsp82) was tested for its ability to direct the expression of the Escherichia coli chloramphenicol acetyltransferase-encoding gene (cat). We report that the level of CAT activity in L. cuprina embryos was comparable to that obtained with the same construct in D. melanogaster, indicating that phsp82 functions efficiently in this non-drosophilid insect. The results suggest that phsp82 may be utilised in other non-drosophilid insects in which poor expression levels are obtained from constructs containing the hsp70 or actin5C promoters.