Rapid Determination of Vitamin B12 by Inductively Coupled Plasma Mass Spectrometry in Multivitamin Tablets
Rapid Determination of Vitamin B12 by Inductively Coupled Plasma Mass Spectrometry in Multivitamin Tablets
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DOI：
10.1080/00387010802371387
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发表时间：
2008-10
影响因子：
1.7
通讯作者：
Ni Zhang;W. Wang;Kuigang Wang;Zhiying Ding;Zeng Wei
中科院分区：
文献类型：
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作者：
Ni Zhang;W. Wang;Kuigang Wang;Zhiying Ding;Zeng Wei
摘要建立了一种快速、简便、灵敏的电感耦合等离子体质谱(ICPMS)测定药物制剂中维生素B12的方法。用5种浓度的维生素B12绘制的校正曲线图呈线性，回归系数R2=0.9994。维生素B12的检出限为0.05 ng/m L。在泵速为30rpm的条件下，维生素B12的分析周期为0.5min，相对标准偏差分别为4.2%、3.5%和2.6%(0.25、2.5和25 ng/mL)。方法用于复合维生素片剂中维生素B12的测定，结果满意。
ABSTRACT In the current work, a rapid, simple, and sensitive inductively coupled plasma mass spectrometry (ICP-MS) method for determining vitamin B12 in pharmaceutical preparations is proposed. The calibration graph plotted with five concentrations of vitamin B12 was linear with a regression coefficient r2 = 0.9994. The detection limit was 0.05 ng/mL for vitamin B12. At a pump rate of 30 rpm, an analysis cycle of vitamin B12, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations of 4.2%, 3.5%, and 2.6% for 0.25 ng/mL, 2.5 ng/mL, and 25 ng/mL, respectively. The method has been applied successfully in the determination of vitamin B12 in multivitamin tablets.