MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection.
MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection.
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MYH9 是猪繁殖与呼吸综合征病毒感染的重要因子。
DOI：
10.1038/srep25120
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发表时间：
2016-04-26
影响因子：
4.6
通讯作者：
Zhou EM
中科院分区：
文献类型：
--
作者：
Gao J;Xiao S;Xiao Y;Wang X;Zhang C;Zhao Q;Nan Y;Huang B;Liu H;Liu N;Lv J;Du T;Sun Y;Mu Y;Wang G;Syed SF;Zhang G;Hiscox JA;Goodfellow I;Zhou EM
猪繁殖与呼吸综合征（PRRS）是由猪繁殖与呼吸综合征病毒（PRRS virus，PRRSV）引起的一种重要的世界性猪病。PRRSV对某些细胞具有有限的嗜性，这可能至少部分归因于在宿主细胞上作为病毒结合或进入的病毒受体或因子的必要细胞分子的表达。然而，这些赋予PRRSV感染的分子尚未完全表征。在这里，我们显示的非肌肉肌球蛋白重链9（MYH 9）作为一个重要因素的PRRSV感染使用抗独特型抗体特异性的PRRSV糖蛋白GP 5的鉴定。MYH 9通过其C-末端结构域与PRRSV GP 5蛋白物理相互作用，并赋予细胞对PRRSV感染的易感性。这些发现表明MYH 9是PRRSV感染的一个重要因素，并为PRRSV-宿主相互作用和病毒进入提供了新的见解，可能有助于制定这种重要猪病的控制策略。
Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is an important swine disease worldwide. PRRSV has a limited tropism for certain cells, which may at least in part be attributed to the expression of the necessary cellular molecules serving as the virus receptors or factors on host cells for virus binding or entry. However, these molecules conferring PRRSV infection have not been fully characterized. Here we show the identification of non-muscle myosin heavy chain 9 (MYH9) as an essential factor for PRRSV infection using the anti-idiotypic antibody specific to the PRRSV glycoprotein GP5. MYH9 physically interacts with the PRRSV GP5 protein via its C-terminal domain and confers susceptibility of cells to PRRSV infection. These findings indicate that MYH9 is an essential factor for PRRSV infection and provide new insights into PRRSV-host interactions and viral entry, potentially facilitating development of control strategies for this important swine disease.