Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC
Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC
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DOI：
10.3892/or_00000804
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发表时间：
2010-06-01
期刊：
影响因子：
4.2
通讯作者：
Itoh, Hiroshi
中科院分区：
文献类型：
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作者：
Kimura, Sotai;Naganuma, Seiji;Itoh, Hiroshi
microRNAs（miRNAs）是一类非编码小分子RNA，通过干扰靶mRNA的翻译来调控细胞增殖和功能。已知miRNA的表达改变可诱导多种人类恶性肿瘤。我们检测了人头颈部鳞状细胞癌（HNSCC）和食管癌（ESCC）中miRNA的表达，并与正常鳞状上皮以及其他器官的恶性肿瘤进行了比较。微阵列分析显示，与正常鳞状上皮细胞系相比，miR-21、miR-16和miR-30 a-5 p在HNSCC和ESCC细胞系中上调，并且miR-205和let-7a在正常和恶性鳞状上皮细胞系中一致高表达。在福尔马林固定石蜡包埋的癌组织和通过激光捕获显微切割获得的配对正常上皮中使用实时定量RT-PCR的验证研究显示，miR-205在恶性和良性鳞状上皮中均显示出最高表达，尽管其在鳞状上皮以外的细胞系和组织中表达较少。miR-21是多种恶性肿瘤中的致癌miRNA，与配对的正常鳞状上皮相比，miR-21在HNSCC和ESCC中也上调。这些结果提示，miR-205可能是正常和恶性鳞状上皮细胞的特异性标志物，而miR-21可能是HNSCC和ESCC的潜在致癌miRNA。
MicroRNAs (miRNAs) are non-coding small RNAs that regulate cell proliferation and functions by interfering with the translation of target mRNAs. Altered expression of miRNA is known to induce various human malignancies. We examined the expression of miRNAs in squamous cell carcinoma of human head and neck (HNSCC) and esophagus (ESCC), compared to that in normal squamous epithelia as well as malignancies of other organs. Microarray analysis showed up-regulation of miR-21, miR-16 and miR-30a-5p in HNSCC and ESCC cell lines compared to normal squamous epithelial cell lines, and consistent high expression of miR-205 and let-7a in both normal and malignant squamous epithelial cell lines. Validation study using real-time quantitative RT-PCR in formalin-fixed paraffin-embedded cancer tissues and paired normal epithelia obtained by Laser-captured microdissection revealed that miR-205 showed highest expression in both malignant and benign squamous epithelia, although it was less expressed in cell lines and tissues other than squamous epithelia. MiR-21, which is an oncogenic miRNA in various malignancies, was also up-regulated in HNSCC and ESCC compared to paired normal squamous epithelia. These results suggest that rniR205 might be a specific marker miRNA of both normal and malignant squamous epithelia, while miR-21 might be a putative oncogenic miRNA in HNSCC and ESCC.