Cytogenetic effects of mycoplasmal infection of cell cultures: a review.
Cytogenetic effects of mycoplasmal infection of cell cultures: a review.
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细胞培养物支原体感染的细胞遗传学效应：综述。
DOI：
10.1007/bf02633326
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Sarama,J
中科院分区：
文献类型：
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作者：
McGarrity,GJ;Vanaman,V;Sarama,J
细胞培养物中支原体的存在代表了真正的体外感染。这种宿主-寄生虫关系受体内感染中的一些因素以及体外培养中的特异性因素的影响，支原体感染的细胞培养物通常比培养的细胞含有多两个对数的支原体。图1显示支原体感染培养物的扫描电子显微照片。Hamet等人{1~估计感染培养物中总蛋白质的25%可能是支原体。支原体在它们感染的培养物中产生广泛的变化并不奇怪。自从罗宾逊等人的第一份报告以来，{2~ 1956年，发表了数百篇关于支原体对其细胞培养宿主的影响的论文。细胞培养支原体的广泛参考书目，其中包括感染的影响，已出版131。本报告的目的是使细胞遗传学家和细胞生物学家了解支原体的一些基本特征，并从已发表的报告或对支原体特征的考虑中综述支原体感染对培养细胞的细胞遗传学的影响。已发表的感染发生率范围为4%至92%{4- 7 L}。感染发生率受所调查的培养物群体、进行支原体测定的频率、细胞培养物的体外寿命以及最重要的是所调查实验室的质量控制程序的影响。已经进行了几次大规模的调查。表1总结了这些情况。我们实验室报告的感染发生率较低可能反映了我们的试验群体包括更多的原代和早期传代材料。
The presence of mycoplasmas in cell cultures represents a true in vitro infection. This hostparasite relationship is influenced by some of the factors operative in in vivo infection as well as those specific for in vitro cultivation, nycoplasmal infected cell cultures generally contain two logs more mycoplasmas than cultured cells. Figure 1 shows a scanning electron micrograph of a mycoplasma infected culture. Hamet et al.{1~ estimate that 25% of the total protein in infected cultures could be mycoplasmal. It is not surprising that mycoplasmas produce extensive changes in cultures they infect. Since the first report of Robinson et al.{2~ in 1956, hundreds of papers have been published on the effects mycoplasmas have on their cell culture hosts. An extensive bibliography of cell culture mycoplasmas, which included effects of infection, has been published 131. The objectives of this report are {al to provide cytogeneticists and cell biologists an understanding of some basic characteristics of mycoplasmas and lb) review the effects that mycoplasma infection has on the cytogenetics of cells in culture from either published reports or consideration of mycoplasmal characteristics. The published incidence of infection has ranged from 4 to 92%{4-7L The incidence is influenced by the population of cultures being surveyed, the frequency with which mycoplasmal assays have been performed, the in vitro life span of the cell cultures, and, most importantly, the quality control programs in the laboratories surveyed. Several large scale surveys have been performed. These are summarized in Table 1. The lower incidences of infection reported from our laboratory probably reflect that our assay population includes more primary and early passage material.