Phenotypic analysis of the chicken thymic microenvironment during ontogenic development.
Phenotypic analysis of the chicken thymic microenvironment during ontogenic development.
复制标题
个体发育过程中鸡胸腺微环境的表型分析。
DOI：
10.1155/1992/32341
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Gershwin,ME
中科院分区：
文献类型：
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作者：
Wilson,TJ;Davidson,NJ;Boyd,RL;Gershwin,ME
与胸腺微环境反应的单克隆抗体（mAb）的发展已经在基质成分中鉴定出不同的亚群，但这些亚区在胸腺内T细胞分化中的功能本质上仍然是一个谜。在本研究中，我们使用这样一组mAb来检查个体发育期间的鸡胸腺，以深入了解这些胸腺区域对T细胞发育不同阶段的贡献，并进一步表征该器官的发育。我们的试剂已经证明了复杂的原始内胚层上皮分化成更专门的结构和其他胸腺基质成分从中外胚层细胞的发展。我们还描述了位于被膜下和血管周围区域的分子，其具有对应于胸腺前体的早期定位和刺激的个体发育表达，以及对应于成熟细胞从胸腺退出的髓质脉管系统上的另一分子。此外，最初表达的两种不同的髓质上皮簇标志物分别对应于髓质中T细胞受体-1（TcR-1）和TcR-2阳性细胞的出现。这些mAb可能代表进一步定义T细胞分化的胸腺调节的优秀试剂。
The development of monoclonal antibodies (mAb) reactive with the thymic microenvironment has identified distinct subpopulations within the stromal component, but the function of these subregions in intrathymic T‐cell differentiation remains essentially an enigma. In this study, we have used such a panel of mAb to examine the chicken thymus during ontogenic development to gain insight into the contributions of these thymic regions to the distinct phases of T‐cell development and to further characterize the development of this organ. Our reagents have demonstrated the complex differentiation of the primitive endodermal epithelium into more specialized structures and the development of other thymic stromal components from mesectodermal cells. We also describe molecules localized to the subcapsular and perivascular regions, which have an ontogenic expression corresponding to the early localization and stimulation of thymic precursors and another molecule on the medullary vasculature expressed corresponding to the exit of mature cells from the thymus. In addition, two markers of distinct medullary epithelial clusters are initially expressed corresponding to the appearance of T‐cell receptor‐1 (TcR‐1) and TcR‐2 positive cells in the medulla, respectively. These mAb potentially represent excellent reagents for further definition of the thymic modulation of T‐cell differentiation.