Genomically recoded organisms expand biological functions.
Genomically recoded organisms expand biological functions.
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DOI：
10.1126/science.1241459
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发表时间：
2013-10-18
期刊：
影响因子：
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通讯作者：
Isaacs FJ
中科院分区：
文献类型：
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作者：
Lajoie MJ;Rovner AJ;Goodman DB;Aerni HR;Haimovich AD;Kuznetsov G;Mercer JA;Wang HH;Carr PA;Mosberg JA;Rohland N;Schultz PG;Jacobson JM;Rinehart J;Church GM;Isaacs FJ
我们描述了一个基因组编码生物（GRO）的结构和特征。我们将大肠杆菌MG1655中已知的所有UAG终止密码子替换为同义的UAA密码子，从而允许删除释放因子1并重新分配UAG翻译功能。这种GRO在非标准氨基酸的掺入方面表现出更好的特性，扩大了体内蛋白质的化学多样性。GRO还表现出对T7噬菌体的抵抗力增强，表明新的遗传密码可以增强病毒的抵抗力。
We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.