Stage-specific onset of condensation and matrix deposition for Meckel's and other first arch cartilages in inbred C57BL/6 mice.
Stage-specific onset of condensation and matrix deposition for Meckel's and other first arch cartilages in inbred C57BL/6 mice.
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近交 C57BL/6 小鼠中梅克尔软骨和其他第一弓软骨的阶段特异性凝结和基质沉积。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
B. Hall
中科院分区：
文献类型：
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作者：
T. Miyake;A. Cameron;B. Hall
凝聚是一个多步骤的过程，涉及细胞募集，细胞与细胞的接触，细胞粘附，并伴随着细胞形状的变化。软骨形成前细胞的浓缩下调和/或灭活细胞增殖，并增强软骨特异性基因的活性。因此，在软骨形成过程中，凝结的发生时间和持续时间是介导细胞和分子事件的重要调节过程。本研究旨在探讨近交系C57 BL/6小鼠第一弓软骨发生凝结和基质形成的时间和持续时间。由于只能在非常精确分期的胚胎中可靠地评估时间，小鼠仅交配2小时，通过体重增加确定妊娠，并基于额鼻区、眼睛、触须、下颌骨和舌骨耳丘（耳廓）和一些内部结构的外部发育将胚胎分配到Theiler [1972]阶段的亚阶段和划分[Miyake et al.，1996年]。使用PNA凝集素组织化学，II型胶原免疫组织化学，马洛里的三色，霍尔和布伦特的四重和甲苯胺蓝染色连续切片的胚胎，和3-D重建的冷凝和基质形成进行了测定。一个单一的，连续的，第一弓软骨形成的冷凝被确定。它由三个组件组成：1）用于联合的喙侧组件; 2）用于Meckel软骨主要部分的核心组件;以及3）用于Meckel软骨尾侧区域和两个耳软骨，锤骨和砧骨的尾侧组件。尾侧成分又进一步分为喙侧和尾侧亚成分，分别形成锤骨和砧骨。细胞排列不同的三个组成部分，可能反映了主轴矩阵的增长。花生凝集素凝集素（PNA）的缩合只有微弱的认可，除非部分预处理与神经氨酸酶，唾液酸的掩蔽作用。冷凝的核心和尾部成分分别出现在第20.12阶段，并在第21.14阶段和第21.32阶段结束。联合部分开始于20.2期，结束于21.32期。硫酸化蛋白聚糖的沉积开始于第21.14阶段的核心组成部分的缩合-之前略有沉积的II型胶原蛋白-并在第21.32阶段的联合和尾部组件。
Condensation is a multistep process, involving cell recruitment, cell-to-cell contact by cell adhesions, and concomitant changes in cell shape. Condensation of prechondrogenic cells down-regulates and/or inactivates cell proliferation and enhances the activities of cartilage-specific genes. Timing of onset and duration of condensation are thus important regulatory processes mediating cellular and molecular events during chondrogenesis. The present study was undertaken to examine timing of onset and duration of condensation and onset of matrix formation for first arch cartilages in inbred C57BL/6 mice. Because timing can only be reliably assessed in very precisely staged embryos, mice were mated for only 2 hr, pregnancy was determined by weight increase, and embryos were assigned to substages and divisions of Theiler's [1972] stages on the basis of external development of the frontonasal area, eyes, vibrissae, mandibular and hyoid auricular hillocks (pinna) and some internal structures [Miyake et al., 1996]. Condensation and matrix formation were determined using PNA lectin histochemistry, type II collagen immunohistochemistry, Mallory's trichrome, Hall and Brunt's quadruple and toluidine blue stains on serially sectioned embryos, and 3-D reconstruction. A single, continuous, first arch chondrogenic condensation was identified. It consisted of three components: 1) a rostral component for the symphysis; 2) a core component for the major portion of Meckel's cartilage; and 3) a caudal component for the caudo-lateral area of Meckel's cartilage and the two ear cartilages, the malleus and incus. The caudal component was further divided into rostral and caudal subcomponents which gave rise to the malleus and incus, respectively. Cellular arrangement differed among the three components, probably reflecting major axes of matrix growth. The condensation was only weakly recognized by peanut agglutinin lectin (PNA), unless sections were pretreated with neuraminidase, showing the masking effects of sialic acid. The core and caudal components of the condensation appeared at stage 20.12 and ended at stage 21.14 and 21.32, respectively. The symphysial component began at stage 20.2 and ended at stage 21.32. Deposition of sulfated proteoglycans began at stage 21.14 in the core component of the condensation--preceding slightly deposition of type II collagen--and at stage 21.32 in the symphysial and caudal components.