A previously undescribed coronavirus associated with respiratory disease in humans
A previously undescribed coronavirus associated with respiratory disease in humans
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DOI：
10.1073/pnas.0400762101
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发表时间：
2004-04-20
影响因子：
11.1
通讯作者：
Osterhaus, ADME
中科院分区：
文献类型：
--
作者：
Fouchier, RAM;Hartwig, NG;Osterhaus, ADME
急性呼吸道疾病的病因有时由于诊断试验的限制或存在尚未确定的病原体而不清楚。在这里，我们描述了一种以前未被识别的冠状病毒的鉴定和特征，该冠状病毒来自一名患有肺炎的8岁男孩。与人类冠状病毒（HCoV） 229E和OC43相比，这种冠状病毒在三级猴肾细胞和Vero细胞中有效复制。结果表明，该冠状病毒与猪流行性腹泻病毒和HCoV 229E亲缘关系最密切。新发现的冠状病毒orf与相关的1组冠状病毒orf的最大氨基酸序列同源性为43% ~ 67%。设计了实时RT-PCR检测方法，以检测先前未描述的冠状病毒在人类中的流行情况。通过这些检测，139名患有病因不明的呼吸系统疾病的患者中有4人（3%）检测到该病毒。所有4例患者均出现发热、流鼻涕和干咳，并且所有4例患者均有潜在或附加发病率。我们的数据将有助于开发诊断测试，以便更详细地研究这种病毒在人类中的流行情况和临床影响。此外，将这种先前未描述的冠状病毒与HCoV 229E和OC43以及严重急性呼吸综合征冠状病毒区分开来也很重要。
The etiology of acute respiratory tract illnesses is sometimes unclear due to limitations of diagnostic tests or the existence of as-yet-unidentified pathogens. Here we describe the identification and characterization of a not previously recognized coronavirus obtained from an 8-mo-old boy suffering from pneumonia. This coronavirus replicated efficiently in tertiary monkey kidney cells and Vero cells, in contrast to human coronaviruses (HCoV) 229E and OC43. The entire cDNA genome sequence of the previously undescribed coronavirus was determined, revealing that it is most closely related to porcine epidemic diarrhea virus and HCoV 229E. The maximum amino acid sequence identity between ORFs of the newly discovered coronavirus and related group 1 coronaviruses ranged from 43% to 67%. Real-time RT-PCR assays were designed to test for the prevalence of the previously undescribed coronavirus in humans. Using these tests, the virus was detected in four of 139 individuals (3%) who were suffering from respiratory illness with unknown etiology. All four patients suffered from fever, runny nose, and dry cough, and all four had underlying or additional morbidity. Our data will enable the development of diagnostic tests to study the prevalence and clinical impact of this virus in humans in more detail. Moreover, it will be important to discriminate this previously undescribed coronavirus from HCoV 229E and OC43 and the severe acute respiratory syndrome coronavirus.