Characterization of transforming viruses rescued from a hamster tumour cell line harbouring the v-src gene flanked by long terminal repeats.
Characterization of transforming viruses rescued from a hamster tumour cell line harbouring the v-src gene flanked by long terminal repeats.
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从含有侧翼长末端重复序列的 v-src 基因的仓鼠肿瘤细胞系中拯救出来的转化病毒的特征。
DOI：
10.1099/0022-1317-67-11-2395
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
Svoboda,J
中科院分区：
文献类型：
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作者：
Geryk,J;Pichrtova,J;Guntaka,RV;Gowda,S;Svoboda,J
研究了从仓鼠肿瘤细胞中拯救的转化病毒感染所产生的前病毒的组织。Southern印迹分析表明，F6细胞株的前病毒结构为长末端重复序列（LTR）-src-LTR，S1定位实验表明该前病毒可能是srcmRNA逆转录后整合而成。在E6细胞系中，前病毒单位排列为LTR-Δgag-src-LTR，表明拯救的转化病毒和辅助病毒之间的重组事件。这些结果表明，转化缺陷病毒只含有thesrc基因可以从非允许的哺乳动物细胞中拯救。
The organization of proviruses derived from infecting transforming viruses rescued from hamster tumour cells was studied. Southern blot analysis indicated that the provirus from the F6 cell line was organized as long terminal repeat (LTR)-src-LTR, and S1 mapping experiments suggested that it was probably derived by reverse transcription ofsrcmRNA followed by integration. In the E6 cell line, the provirus unit was arranged as LTR-Δgag-src-LTR, indicating a recombination event between the rescued transforming virus and the helper virus. These results suggest that transforming defective viruses containing only thesrcgene can be rescued from non-permissive mammalian cells.