AFM study of interaction forces in supported planar DPPC bilayers in the presence of general anesthetic halothane
AFM study of interaction forces in supported planar DPPC bilayers in the presence of general anesthetic halothane
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DOI：
10.1016/j.bbamem.2006.02.033
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发表时间：
2006-04-01
影响因子：
3.4
通讯作者：
Cramb, D.
中科院分区：
文献类型：
--
作者：
Leonenko, Z.;Finot, E.;Cramb, D.
尽管进行了大量的研究，但全身麻醉药作用的分子机制仍不清楚。已经表明，麻醉剂效力与麻醉剂分配到膜中的能力有关。我们研究了在全身麻醉剂氟烷存在下，支持的二棕榈酰磷脂酰胆碱（DPPC）双层的结构，动力学和相互作用力的变化。在本研究中，我们使用原子力显微镜测量探针和双层之间的相互作用力。分析了力曲线的变化作为麻醉剂掺入的函数。力测量与AFM成像数据吻合良好，并提供了有价值的信息双层厚度，结构转变，和氟烷引起的静电和粘附性能的变化。(c)2006 Elsevier B.V.保留所有权利。
In spite of numerous investigations, the molecular mechanism of general anesthetics action is still not well understood. It has been shown that the anesthetic potency is related to the ability of an anesthetic to partition into the membrane. We have investigated changes in structure, dynamics and forces of interaction in supported dipalmitoylphosphatidylcholine (DPPC) bilayers in the presence of the general anesthetic halothane. In the present study, we measured the forces of interaction between the probe and the bilayer using an atomic force microscope. The changes in force curves as a function of anesthetic incorporation were analyzed. Force measurements were in good agreement with AFM imaging data, and provided valuable information on bilayer thickness, structural transitions, and halothane-induced changes in electrostatic and adhesive properties. (c) 2006 Elsevier B.V. All rights reserved.