Recent advances in the bioremediation of persistent organic pollutants via biomolecular engineering
Recent advances in the bioremediation of persistent organic pollutants via biomolecular engineering
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DOI：
10.1016/j.enzmictec.2004.07.024
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发表时间：
2005-10-03
影响因子：
3.4
通讯作者：
Obbard, JP
中科院分区：
文献类型：
--
作者：
Ang, EL;Zhao, HM;Obbard, JP
随着生物分子工程的发展，利用转基因微生物对持久性有机污染物（POPs）进行生物修复已成为环境保护领域的一个快速发展的研究领域。两种主要的生物分子方法，合理设计和定向进化，已开发出工程增强微生物和酶的持久性有机污染物的生物降解。本文综述了近年来生物分子工具在提高微生物修复三大类持久性有机污染物（POPs）--多环芳烃（PAHs）、多氯联苯（PCBs）和农药的能力方面的最新进展和应用。大多数实例侧重于重新设计参与持久性有机污染物生物修复的酶的各种功能，包括酶的表达水平、酶活性和底物特异性。总的来说，生物分子工程技术的潜力迅速扩大，创造了令人兴奋的潜力，修复环境中一些最难降解和危险的化合物。(c)2005年爱思唯尔公司All rights reserved.
With recent advances in biomolecular engineering, the bioremediation of persistent organic pollutants (POPs) using genetically modified microorganisms has become a rapidly growing area of research for environmental protection. Two main biomolecular approaches, rational design and directed evolution, have been developed to engineer enhanced microorganisms and enzymes for the biodegradation of POPs. This review describes the most recent developments and applications of these biomolecular tools for enhancing the capability of microorganisms to bioremediate three major classes of POPs - polycyclic aromatic hydrocabons (PAHs), polychlorinated biphenyls (PCBs) and pesticides. Most of the examples focused on the redesign of various features of the enzymes involved in the bioremediation of POPs, including the enzyme expression level, enzymatic activity and substrate specificity. Overall, the rapidly expanding potential of biomolecular engineering techniques has created the exciting potential of remediating some of the most recalcitrant and hazardous compounds in the environment. (c) 2005 Elsevier Inc. All rights reserved.