Extraction by Microwave-assisted Hydro-distillation and GC-MS Analysis of Volatile Oil from Artemisia Rubripes Nakai
Extraction by Microwave-assisted Hydro-distillation and GC-MS Analysis of Volatile Oil from Artemisia Rubripes Nakai
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发表时间：
2014
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通讯作者：
Xiao Donghai;Li Xia;Dong Xinrong;Cui Fuzhi;Sun Yuqiong
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作者：
Xiao Donghai;Li Xia;Dong Xinrong;Cui Fuzhi;Sun Yuqiong
目的建立微波辅助水蒸馏法（MAE-HD）提取红叶蒿挥发油的方法。方法采用先单因素再正交阵列设计的方法优化提取条件。并采用气相色谱-质谱联用仪(GC-MS)对样品的化学成分进行了分析。结果MAE-HD的最佳提取条件为微波功率480 W、料液比1 10、水浸时间4 h。在此条件下，29 min 所得挥发油收率为 0.29%，明显高于加氢蒸馏（HD）3 h 所得挥发油收率 0.23%。 GC-MS 鉴定了 61 种化合物。挥发油主要成分为石竹烯(13.85%)、二甘醇(11.51%)、桉树脑(9.84%)、氧化石竹烯(4.80%)、香叶烯D(3.99%)、α-硒烯(3.47%)、1-萜品烯-4-醇(2.50%)、 eudesm-7(11)en-4-ol(2.50%)。与HD相比，挥发油的主要成分及相对含量基本相似，但也存在一定差异。结论 MAE-HD具有高效的优点，适用于红叶蒿挥发油的提取。
OBJECTIVE To develop the method of extraction of volatile oil from Artemisia rubripes Nakai by microwave-assisted hydro-distillation (MAE-HD). METHODS The extraction conditions were optimized by single factor firstly and then orthogonal array design methods. And the chemical compositions of samples were analyzed by gas chromatography-mass spectrometry(GC-MS). RESULTS The optimal extraction conditions of MAE-HD were microwave power at 480 W, solid/liquid ratio at 1 10, and water-soaked time for 4 h. Under this condition, the yield of volatile oil obtained was 0.29% for 29 min, which was obviously higher than that of 0.23% obtained by hydro-distillation (HD) for 3 h. Sixty-one compounds had been identified by GC-MS. The main components of volatile oil were caryophyllene(13.85%), alcanfor(11.51%), cineole(9.84%), caryophyllene oxide(4.80%), germacrene D(3.99%), α-selinene (3.47%), 1-terpinen-4-ol(2.50%), eudesm-7(11)en-4-ol(2.50%). Compared with HD, the main constituents and the relative contents of volatile oil were basically similar, but there were also some differences between them. CONCLUSION MAE-HD has the advantage of efficiency, and it’s suitable for the extraction of volatile oil from Artemisia rubripes Nakai.