Ontogeny of the digestive enzyme activity of the Amazonian pimelodid catfish Pseudoplatystoma punctifer (Castelnau, 1855)
Ontogeny of the digestive enzyme activity of the Amazonian pimelodid catfish Pseudoplatystoma punctifer (Castelnau, 1855)
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亚马逊鲮鱼消化酶活性的本体发育
DOI：
10.1016/j.aquaculture.2019.01.059
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发表时间：
2019-04-15
期刊：
影响因子：
4.5
通讯作者：
Darias, Maria J.
中科院分区：
文献类型：
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作者：
Castro-Ruiz, Diana;Mozanzadeh, Mansour Torfi;Darias, Maria J.
本研究的目的是通过分析主要肠酶（碱性磷酸酶、氨肽酶 N、麦芽糖酶和亮氨酸丙氨酸肽酶）、胰酶（胰蛋白酶、胰凝乳蛋白酶、总碱性蛋白酶、胆盐活化脂肪酶和淀粉酶）和胃酶（胃蛋白酶）的活性，评估 Pseudoplatystoma punctifer 消化系统的功能个体发育。受精后(dpf)4至27天，在再循环系统中以90只幼虫L-1的初始密度、27.8+/-0.7℃和0L：24D光周期，一式三份地饲养幼虫。幼虫从 4 至 17 dpf 饲喂贫血无节幼体，并在 3 天内断奶至实验配制的饲料（粗蛋白含量类似于 45%；粗脂肪含量类似于 10%；粗碳水化合物类似于 8%），然后继续采用相同的饮食直至试验结束。 P. punctifer 显示出指数生长模式，具有两种不同的生长速率：一种是从孵化到 12 dpf 的较慢生长速率，另一种是从 12 dpf 到 27 dpf 的较快生长速率。从孵化开始就检测胰酶和肠酶的比活性和总活性。消化系统在 12 dpf 时开始发挥作用，表明从幼虫到幼虫阶段的过渡（碱性消化到酸性消化）。因此，从那天起，人们就可以断奶了。 17 dpf 后观察到的酶活性变化反映了酶机械对所提供的新饮食的适应。 P. punctifer 幼虫表现出快速的消化系统发育，具有热带和肉食物种典型的酶谱。
The aim of the study was to evaluate the functional ontogeny of the digestive system of Pseudoplatystoma punctifer through the analysis of the activity of the main intestinal (alkaline phosphatase, aminopeptidase N, maltase and leucine-alanine peptidase), pancreatic (trypsin, chymotrypsin, total alkaline proteases, bile-salt activated lipase and amylase) and gastric (pepsin) enzymes. Larvae were raised in triplicate in a recirculation system from 4 to 27 days post fertilization (dpf) at an initial density of 90 larvae L-1 , 27.8 +/- 0.7 degrees C and 0 L: 24D photoperiod. Larvae were fed from 4 to 17 dpf with Anemia nauplii and weaned onto an experimentally formulated feed (crude protein content similar to 45%; crude fat content similar to 10%; crude carbohydrate similar to 8%) within 3 days, then continued with the same diet until the end of the trial. P. punctifer showed an exponential growth pattern with two different growth rates: a slower one from hatching to 12 dpf followed by a faster one from 12 to 27 dpf. The specific and total activities of the pancreatic and intestinal enzymes were detected from hatching. The digestive system was functional at 12 dpf, indicating the transition from the larval to the juvenile stage (alkaline to acid digestion). Therefore individuals could be weaned from that day onwards. The variations observed in the enzymatic activity from 17 dpf reflected the adaptation of the enzymatic machinery to the new diet supplied. P. punctifer larvae showed a fast digestive system development with an enzymatic profile typical of a tropical and carnivorous species.