The Nile River Microbiome Reveals a Remarkably Stable Community Between Wet and Dry Seasons, and Sampling Sites, in a Large Urban Metropolis (Cairo, Egypt)
The Nile River Microbiome Reveals a Remarkably Stable Community Between Wet and Dry Seasons, and Sampling Sites, in a Large Urban Metropolis (Cairo, Egypt)
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DOI：
10.1089/omi.2018.0090
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发表时间：
2018-08-01
影响因子：
3.3
通讯作者：
Yassin, Aymen S.
中科院分区：
文献类型：
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作者：
Eraqi, Walaa A.;ElRakaiby, Marwa T.;Yassin, Aymen S.
世界淡水供应需要微生物多样性分析，作为未来生态研究的第一步，并监测水的安全和质量。尼罗河是非洲一条主要的北流河流，其水质在空间和时间上都有变化。在这里，我们提出了两个季节的尼罗河水的第一个微生物组分析：（1）夏季代表雨季，（2）冬季代表旱季，在埃及首都开罗周围采样。地表河水样品采集自河流路径沿线沿着选定的地点，并通过16 S rRNA基因的下一代测序分析微生物组成。我们发现尼罗河微生物群落结构沿着所研究的地理城市站点以及在雨季和旱季之间具有惊人的稳定性，所有样品中共享操作分类单位值的比例很高。该群落以蓝细菌（主要是聚球藻）、放线菌候选家族（ACK-M1）和变形菌（主要是丛枝藻科）为主。在这些优势类群中，聚球藻属的相对丰度表现出季节性变化。其他类群在所有季节和地点都非常罕见，包括被认为是病原体的属成员，如不动杆菌属、气单胞菌属和军团菌属。此外，与世界其他地区淡水微生物组数据的比较表明，大河地表水群落的变化有限。我们的研究结果提供了第一个洞察微生物组成的一个最著名的河流附近的一个大大都会。
World freshwater supplies are in need of microbiome diversity analyses as a first step to future ecological studies, and to monitor water safety and quality. The Nile is a major north-flowing river in Africa that displays both spatial and temporal variations in its water quality. Here, we present the first microbiome analysis of the Nile River water in two seasons: (1) summer representing the wet season, and (2) winter representing the dry season, as sampled around Cairo, the capital of Egypt. Surface river water samples were collected from selected locations along the path of river, and the microbial composition was analyzed by next-generation sequencing of the 16S rRNA gene. We found a striking stability in the Nile microbiome community structure along the examined geographical urban sites and between the wet and dry seasons as evidenced by the high proportion of shared operational taxonomic unit values among all samples. The community was dominated by the Cyanobacteria (mainly Synechococcus), Actinobacteria candidate family (ACK-M1), and Proteobacteria (mainly family Comamonadaceae). Among these dominant taxa, Synechococcus exhibited seasonal driven variation in relative abundance. Other taxa were predominantly rare across all seasons and locations, including genera members of which have been implicated as pathogens such as Acinetobacter, Aeromonas, and Legionella. In addition, comparisons with data on freshwater microbiome in other world regions suggest that surface water communities in large rivers exhibit limited variation. Our results offer the first insights on microbial composition in one of the most notable rivers near a large metropolis.