Characterization of functional vanilloid receptors expressed by mast cells
Characterization of functional vanilloid receptors expressed by mast cells
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DOI：
10.1182/blood.v91.4.1332
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发表时间：
1998-02-15
期刊：
影响因子：
20.3
通讯作者：
Blumberg, PM
中科院分区：
文献类型：
--
作者：
Bíró, T;Maurer, M;Blumberg, PM
辣椒素及其强效类似物树脂干扰素（RTX）通过特定的香草样受体作用于感觉神经元。c型受体与Ca-45摄取偶联，而r型受体通过[H-3]RTX结合检测。我们在此描述了小鼠肥大细胞（MCs）中特定的香草样蛋白反应。在MC系和骨髓来源的肥大细胞中。辣椒素和RTX诱导Ca-45摄取与培养大鼠背根神经节神经元（DRGs）相似。这种反应被拮抗剂辣椒平和钌红拮抗。与DRGs一样，用辣椒素或RTX预处理MCs诱导脱敏，随后刺激Ca-45的摄取。RTX在MCs中的脱敏效力与Ca-45摄取的脱敏效力一致，而在DRGs中，脱敏效力明显低于高亲和力[H-3]RTX结合位点。与这种差异一致的是，在MCs中，我们无法检测到[H-3]RTX结合。与DRGs相比，香草素对MCs无细胞毒性。虽然香草素不会引起MCs的脱粒，但在P815克隆中，辣椒素诱发了选择性的白介素-4释放。我们得出结论，某些MCs具有香草受体，但只有c型作为通道。我们发现MCs可以直接对辣椒素作出反应，这需要重新评估对香草素作出反应的体内炎症途径。这是美国政府的工作。它的使用没有限制。
Capsaicin and its ultrapotent analog resiniferatoxin (RTX) act through specific vanilloid receptors on sensory neurons. The C-type receptor is coupled to Ca-45 uptake, whereas the R-type is detectable by [H-3]RTX binding. We describe here specific vanilloid responses in murine mast cells (MCs). In the MC lines and in bone marrow-derived mast cells. capsaicin and RTX induced Ca-45 uptake similarly to that observed for cultured rat dorsal root ganglion neurons (DRGs). This response was antagonized by the antagonists capsazepine and ruthenium red. As in DRGs, pretreatment of MCs with capsaicin or RTX induced desensitization to subsequent stimulation of Ca-45 uptake. The potency for desensitization by RTX in the MCs corresponded to that for Ca-45 uptake, whereas in DRGs it occurred at significantly lower concentrations corresponding to that for the high-affinity [H-3]RTX binding site. Consistent with this difference, in MCs we were unable to detect [H-3]RTX binding. Vanilloids were noncytotoxic to the MCs, in contrast to the DRGs. Although vanilloids did not cause degranulation in MCs, in the P815 clone capsaicin evoked selective interleukin-4 release. We conclude that certain MCs possess vanilloid receptors, but only the C-type that functions as a channel. Our finding that MCs can respond directly to capsaicin necessitates a reevaluation of the in vivo pathway of inflammation in response to vanilloids. This is a US government work. There are no restrictions on its use.