Enhancement of Interleukin 2 Production in Human and Gibbon T Cells after In Vitro Treatment with Lithium
Enhancement of Interleukin 2 Production in Human and Gibbon T Cells after In Vitro Treatment with Lithium
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锂体外处理后人类和长臂猿 T 细胞中白介素 2 产量的增强
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
Xuhao Yang
中科院分区：
文献类型：
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作者：
Yiyuan Wu;Xuhao Yang
本文研究了微量元素锂、硒、锌对人外周血单个核细胞(PBMC)、MLA144和Jurkat细胞产生白介素2(IL-2)的影响。锂显著促进MLA144和PBMC产生IL-2，但对Jurkat无明显影响。硒只对MLA144产生IL-2有促进作用，而锌对上述三个系统中IL-2的产生均无影响。锂对IL-2产生的促进作用与十四酰佛波醇醋酸酯(TPA)的不同之处在于：(1)TPA能逆转抗CD2单抗对IL-2产生的抑制作用，而锂则不能；(2)锂不能与抗CD3单抗协同诱导IL-2产生。锂对IL-2产生的影响处于淋巴细胞活化的早期阶段。在植物血凝素刺激的PBMC培养中加入霍乱毒素或茶碱可抑制IL-2的产生。然而，IL-2的产生可以通过锂来恢复。霍乱毒素或茶碱处理的PBMC中cAMP相应增加，锂可逆转这一作用。因此，锂通过减少cAMP来恢复IL-2的产生。
Abstract The effect of trace elements lithium, selenium, and zinc on interleukin 2 (IL-2) production by peripheral blood mononuclear cells (PBMC), MLA144, and Jurkat cell lines has been studied. Lithium markedly enhanced IL-2 production by MLA144 and PBMC, but not by Jurkat. Selenium could only enhance IL-2 production by MLA144, whereas in none of these three systems was IL-2 production altered by zinc. The enhancing effect of lithium on IL-2 production showed some differences from that of tetradecanoylphorbol acetate (TPA) in the following aspects: (i) TPA could reverse the inhibitory effect of anti-CD2 monoclonal antibody on IL-2 production, whereas lithium could not; and (ii) lithium was unable to synergistically induce IL-2 production with anti-CD3 monoclonal antibody as TPA did. The effect of lithium on IL-2 production was in the early phase of lymphocyte activation. The addition of cholera toxin or theophylline to phytohemagglutinin-stimulated PBMC culture suppressed IL-2 production. However, IL-2 production could be restored by lithium. There was a corresponding increase in cAMP in cholera toxin or theophylline-treated PBMC, which could be reversed by lithium. Therefore, lithium restores IL-2 production via a decrease in cAMP.
T 细胞对 Con A 的反应对辅助细胞的条件要求。
DOI：
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发表时间：
1986
期刊：
Lymphokine research
影响因子：
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作者：
Hoffmann,MK;Chun,M;Hirst,JA
通讯作者：
Hirst,JA