Isolation and characterization of synovial cells from the human temporomandibular joint
Isolation and characterization of synovial cells from the human temporomandibular joint
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DOI：
10.1111/j.1600-0714.2006.00369.x
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发表时间：
2006-02-01
影响因子：
3.3
通讯作者：
Fukuda, M
中科院分区：
文献类型：
--
作者：
Nagai, H;Miyamoto, Y;Fukuda, M
背景技术背景：颞下颌关节紊乱病（TMDs）患者的滑膜组织常表现为慢性炎症改变，滑膜细胞参与了TMDs的发病过程。滑膜由滑膜衬里层和结缔组织衬里下层组成。滑膜衬里层由两种细胞组成：巨噬细胞样A型细胞和成纤维细胞B型细胞。本研究的目的是从人颞下颌关节（TMJ）滑膜细胞的分离和鉴定。结果：SGA 2细胞表达成纤维细胞标志物波形蛋白和脯氨酰4-羟化酶，还表达层粘连蛋白和热休克蛋白27，这些都是B型细胞的标志物。然而，一些细胞表达巨噬细胞标志物CD 68。这些CD 68阳性细胞同时表达层粘连蛋白。结论：我们分离和培养的滑膜型B细胞从人颞下颌关节，并确定存在的中间型滑膜衬里细胞，具有A型和B型细胞的表型特性，滑膜衬里细胞。
BACKGROUND: The synovial tissues with temporomandibular disorders (TMDs) often show chronic inflammatory changes and the synovial cells participate in the pathogenic processes of TMDs. The synovial membrane is composed of a synovial lining layer and a connective sublining layer. The synovial lining layer is made up of two kinds of cells: macrophage-like type A and fibroblastic type B cells. The aim of this study was to isolate and characterize synovial cells from the human temporomandibular joint (TMJ).METHODS: Synovial cells were isolated using an explant culture method. Then, we characterized the cultured synovial cells (SGA2 cells) using immunocytochemistry.RESULTS: SGA2 cells expressed the fibroblastic markers vimentin and prolyl 4-hydroxylase; they also expressed laminin and heat shock protein 27, all of which are markers of type B cells. However, some cells expressed the macrophage marker CD68. These CD68-positive cells simultaneously expressed laminin.CONCLUSIONS: We isolated and cultured synovial type B cells from the human TMJ, and identified the presence of intermediate type synovial lining cells, having the phenotypic properties of both type A and type B cells, among the synovial lining cells.