Visual degumming process of ramie fiber using a microbial consortium RAMCD407
Visual degumming process of ramie fiber using a microbial consortium RAMCD407
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DOI：
10.1007/s10570-019-02288-1
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发表时间：
2019-03
期刊：
影响因子：
5.7
通讯作者：
Kewei Mao;Honggao Chen;Hanghang Qi;Zidong Qiu;Li Zhang;Jiangang Zhou
中科院分区：
文献类型：
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作者：
Kewei Mao;Honggao Chen;Hanghang Qi;Zidong Qiu;Li Zhang;Jiangang Zhou
采用光学显微镜、偏光显微镜、荧光显微镜和扫描电子显微镜结合酶活性分析等技术，研究了RAMCD407对苎麻脱胶过程中的形态变化和生化过程。结果表明，脱胶在56h内完成，然后用0.2%的NaOH处理。最终纤维的残胶率为2.84%，断裂强度为5.2 cN/dtex。满足了对麻纺的要求。脱胶包括四个主要过程：吸水和形成CCCO；去除皮层；去除中层胶体；去除纤维表面的胶体。前两个过程是在低酶活条件下完成的，第三个过程与较高的果胶酶活力有关，最后一个过程与较高的果胶酶和木聚糖酶活力有关。事实上，只需要水解一小部分半纤维素和果胶就能确保纤维分离。在此过程中，果胶酶在木聚糖酶的支持下发挥了关键作用。通过过滤和/或离心法从残液中回收有价值的脱胶残留物，如果胶酶结晶、果胶块、木聚糖片和微晶纤维素。这些废渣的再利用有助于提高苎麻行业的整体经济效益。脱胶过程中出现了具有双壳结构的球状结构，扫描电子显微镜显示其外壳由富含木聚糖的物质组成。与传统的化学脱胶方法相比，RAMCD407脱胶是一种环保型脱胶技术。
AbstractMorphological changes and biochemical process of ramie retting by RAMCD407 was investigated using microscopy techniques, including OM, PLM, FLSM and SEM coupled with enzyme activity analysis. Results showed that retting was completed within 56 h followed by 0.2% NaOH treatment. Residual gum content and breaking strength of the final fiber was 2.84% and 5.2 cN/dtex, respectively. This fulfills the requirement for ramie spinning. Retting included four main processes: water absorption and CCCO formation; cortex removal; removal of gum in the middle lamella; and removal of gum on the surface of fiber. The first two processes were completed under low enzyme activity condition, while the third process was related to higher pectinase enzyme activity and the last process was related to higher enzyme activities of pectinase and xylanase. In fact, only a small fraction of hemicellulose and pectin is required to be hydrolyzed to ensure fiber separation. In this process, pectinase played a key role supported by xylanase. Valuable retting residues, such as pectinase crystals, pectin blocks, xylan chips and microcrystalline cellulose was recovered from residual liquor by filtration and/or centrifugation. Reuse of these waste residues can help increase the overall economic benefit for ramie industries. The occurrence of spherical balls with double shell structure was found in retting process, and FLSM showed that the outer shell was composed of xylan-rich material. Compared to traditional chemical degumming methods, ramie retting by RAMCD407 is an eco-friendly degumming technique.Graphical abstract