Gold Nanoclusters@Ru(bpy)₃²⁺-Layered Double Hydroxide Ultrathin Film as a Cathodic Electrochemiluminescence Resonance Energy Transfer Probe.
Gold Nanoclusters@Ru(bpy)₃²⁺-Layered Double Hydroxide Ultrathin Film as a Cathodic Electrochemiluminescence Resonance Energy Transfer Probe.
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DOI：
10.1021/acs.analchem.5b02208
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发表时间：
2015-07
影响因子：
7.4
通讯作者：
Yingchang Yu;Chao Lu;Meining Zhang
中科院分区：
文献类型：
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作者：
Yingchang Yu;Chao Lu;Meining Zhang
本文首次报道了在Au电极上，用混合蓝色bsa -金纳米团簇（AuNCs）和Ru(bpy)3(2+)（表示为AuNCs@Ru）的煤层状双氢氧化物（LDH）纳米片逐层（LBL）静电组装制备阴极电化学发光（ECL）共振能量转移（ERET）系统。可能的ECL机制表明，煤- ldh纳米片的出现在Au电极上产生了AuNCs@Ru的长程堆积顺序，促进了制备的AuNCs@Ru-LDH超薄膜（utf）上BSA-AuNC供体和Ru(bpy)3（2+）受体之间的ERET的发生。此外，还观察到6-巯基嘌呤（6-MP）在2.5 ~ 100 nM的线性范围内可以有效地淬灭阴极ECL强度，检出限为1.0 nM。基于这些有趣的现象，简易阴极ECL传感器成功地将6-MP与人类血清和尿液样本中的其他含硫醇化合物（如半胱氨酸和谷胱甘肽）区分出来。所提出的传感方案为利用层状utf作为Ru(bpy)3（2+）阴极ECL的平台开辟了一条道路。
Herein, it is the first report that a cathodic electrochemiluminescence (ECL) resonance energy transfer (ERET) system is fabricated by layer-by-layer (LBL) electrostatic assembly of CoAl layered double hydroxide (LDH) nanosheets with a mixture of blue BSA-gold nanoclusters (AuNCs) and Ru(bpy)3(2+) (denoted as AuNCs@Ru) on an Au electrode. The possible ECL mechanism indicates that the appearance of CoAl-LDH nanosheets generates a long-range stacking order of the AuNCs@Ru on an Au electrode, facilitating the occurrence of the ERET between BSA-AuNC donors and Ru(bpy)3(2+) acceptors on the as-prepared AuNCs@Ru-LDH ultrathin films (UTFs). Furthermore, it is observed that the cathodic ECL intensity can be quenched efficiently in the presence of 6-mercaptopurine (6-MP) in a linear range of 2.5-100 nM with a detection limit of 1.0 nM. On the basis of these interesting phenomena, a facile cathodic ECL sensor has successfully distinguished 6-MP from other thiol-containing compounds (e.g., cysteine and glutathione) in human serum and urine samples. The proposed sensing scheme opens a way for employing the layered UTFs as a platform for the cathodic ECL of Ru(bpy)3(2+).