Selenium protects wheat seedlings against salt stress-mediated oxidative damage by up-regulating antioxidants and osmolytes metabolism
Selenium protects wheat seedlings against salt stress-mediated oxidative damage by up-regulating antioxidants and osmolytes metabolism
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DOI：
10.1016/j.plaphy.2019.02.004
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发表时间：
2019-04-01
影响因子：
6.5
通讯作者：
El-Esawi, Mohamed A.
中科院分区：
文献类型：
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作者：
Elkelish, Amr A.;Soliman, Mona H.;El-Esawi, Mohamed A.
盐胁迫阻碍了大多数作物的生长，并大大降低了产量。因此，本试验以小麦（Trideum aestivum L.）研究了补硒（5和10 μ M Se）对提高植物耐盐性的作用。暴露于盐度（100 mM NaCl）减少的长度，新鲜和干生物量产量方面的增长。硒是有效的改善Nag应力的有害影响显着水平时，提供在5 μ M的浓度相比，10 μ M。5 μ M硒浓度的应用没有表现出显着的生理生化参数的影响。植物补充5 μ M硒表现出最高的RWC，叶绿素合成，光合作用。硒的补充减少了NaCl介导的氧化损伤，通过上调抗氧化系统的酶组分的活性和抗坏血酸和谷胱甘肽的积累。此外，5 μ M硒证明有利于提高脯氨酸和糖的积累在正常和NaCl胁迫的幼苗提供额外的渗透压，以维持RWC和保护光合作用。硒还通过调节γ-谷氨酰激酶（γ-GK）和脯氨酸氧化酶（PROX）的活性影响脯氨酸代谢，导致其合成增加和降解减少。此外，硒降低了Na/K比值，也提高了氮和钙的吸收。总之，低浓度的硒可以有益于防止盐介导的损伤，需要进一步的研究来解开潜在的机制。
Salinity stress hampers the growth of most crop plants and reduces yield considerably. Therefore, experiments were conducted on wheat (Trideum aestivurn L.) plants for studying the role of selenium (5 and 10 mu M Se) supplementation in strengthening the salinity stress tolerance. Exposure to salinity (100 mM NaCl) reduced growth in terms of length, fresh and dry biomass yield. Se was affective in ameliorating the deleterious effects of Nag stress to significant levels when supplied at 5 mu M concentrations compared to 10 mu M. Application of Se at 5 mu M concentration did not show significant impacts on the physiological and biochemical parameters studied. Plants supplemented with 5 mu M Se exhibited the highest RWC, chlorophyll synthesis, and photosynthesis. Se supplementation reduced the NaCl-mediated oxidative damage by up-regulating the activity of enzymatic components of the antioxidant system and the accumulation of ascorbate and glutathione. Furthermore, 5 mu M Se proved beneficial in enhancing proline and sugar accumulation in normal and NaCl-stressed seedlings providing extra osmolarity to maintain RWC and protect photosynthesis. Se also affected proline metabolism by modulating the activities of the gamma-glutamyl kinase (gamma-GK) and proline oxidase (PROX) leading to its greater synthesis and lesser degradation. Moreover, it was observed that Se declined the Na/K ratio and also improved nitrogen and Ca uptake. Conclusively, Se at low concentration can be beneficial in preventing salinity-mediated damage and further studies are required to unravel underlying mechanisms.