INVITRO SEPARATION OF CHIMERAL PEARS INTO THEIR COMPONENT GENOTYPES
INVITRO SEPARATION OF CHIMERAL PEARS INTO THEIR COMPONENT GENOTYPES
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DOI：
10.1007/bf00037199
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发表时间：
1990-07-01
期刊：
影响因子：
1.9
通讯作者：
CHEVREAU, E
中科院分区：
文献类型：
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作者：
ABUQAOUD, H;SKIRVIN, RM;CHEVREAU, E
利用梨不定芽再生体系，将两个嵌合体梨分离成其组成基因型。这两个品种是一个杂色的类型，“路易丝邦尼Panachee”和一个红色的果实突变体，“红色哈代”。将这些栽培品种的叶置于由Nitsch和Nitsch（1969）盐组成的再生培养基上，所述Nitsch和Nitsch（1969）盐提供有不同水平的噻苯隆（TDZ）和NAA。两个月后，将再生体转移到Lepoivre盐的增殖培养基上。后来他们被评估为嵌合状态。杂色型再生植株100%分离，多数植株为绿色，少数植株为白化病。再生是更有效地解离杂色嵌合体比快速芽增殖和物理损伤。在“Red Hardy”中，在再生培养基上两个月后，20 - 33%的再生体是绿色，其余的是红色。红色和绿色再生体的稳定性在补充有各种水平的蔗糖的培养基上和通过总花色素苷测量来评估。两种类型都很稳定。
An adventitious shoot regeneration system for Pyrus communis was used to separate two chimeral pears into their component genotypes. The two cultivars were a variegated type, ''Louise Bonne Panachee'' and a red fruited mutant, ''Red Hardy''. Leaves of these cultivars were placed onto a regeneration medium consisting of Nitsch and Nitsch (1969) salts supplied with various levels of Thidiazuron (TDZ) and NAA. After two months the regenerants were moved onto a proliferation medium of Lepoivre salts. Later they were evaluated for their chimeral status. Among the regenerants of the variegated type, 100% segregation occurred, most shoots were green, a few were albino. Regeneration was more efficient for dissociating the variegated chimera than rapid shoot multiplication and physical injury. In ''Red Hardy'', after two months on the regeneration medium, 20 to 33% of the regenerants were green, the rest were red. The stability of the red and the green regenerants were assessed on media supplemented with various levels of sucrose and by total anthocyanins measurement. Both types were stable.