Vitamin K2 Prevents Glucocorticoid-induced Osteonecrosis of the Femoral Head in Rats.
Vitamin K2 Prevents Glucocorticoid-induced Osteonecrosis of the Femoral Head in Rats.
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DOI：
10.7150/ijbs.13269
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发表时间：
2016
影响因子：
9.2
通讯作者：
Gao YS
中科院分区：
文献类型：
--
作者：
Zhang YL;Yin JH;Ding H;Zhang W;Zhang CQ;Gao YS
糖皮质激素药物是非创伤性股骨头坏死 (ONFH) 的最常见原因之一，而维生素 K2 (VK2) 已被证明在骨代谢中发挥重要且有益的作用。在这项研究中，我们假设 VK2 可以降低大鼠模型中糖皮质激素诱导的 ONFH 的发生率。通过体外研究，我们研究了骨髓干细胞在甲基强的松龙存在下如何增殖和分化，特别检查了成骨相关蛋白，包括 Runx2、碱性磷酸酶和骨钙素。通过体内研究，我们在大鼠中建立了糖皮质激素诱导的 ONFH 并研究了 VK2 的预防作用。我们采用显微 CT 扫描、股骨头血管造影以及组织学和免疫组织化学分析，证明 VK2 对软骨下骨小梁产生有益作用。总之，VK2 是糖皮质激素对成骨祖细胞的有效拮抗剂。其潜在机制包括加速 BMSC 增殖和促进骨形成相关蛋白表达，两者相结合有助于预防大鼠糖皮质激素诱导的 ONFH。
Glucocorticoid medication is one of the most common causes of atraumatic osteonecrosis of the femoral head (ONFH), and vitamin K2 (VK2) has been shown to play an important and beneficial role in bone metabolism. In this study, we hypothesized that VK2 could decrease the incidence of glucocorticoid-induced ONFH in a rat model. Using in vitro studies, we investigated how bone marrow-derived stem cells in the presence of methylprednisolone proliferate and differentiate, specifically examining osteogenic-related proteins, including Runx2, alkaline phosphatase and osteocalcin. Using in vivo studies, we established glucocorticoid-induced ONFH in rats and investigated the preventive effect of VK2. We employed micro-CT scanning, angiography of the femoral head, and histological and immunohistochemical analyses, which demonstrated that VK2 yielded beneficial effects for subchondral bone trabecula. In conclusion, VK2 is an effective antagonist for glucocorticoid on osteogenic progenitors. The underlying mechanisms include acceleration of BMSC propagation and promotion of bone formation-associated protein expression, which combine and contribute to the prevention of glucocorticoid-induced ONFH in rats.