Engineered PQQ-Glucose Dehydrogenase as a Universal Biosensor Platform
Engineered PQQ-Glucose Dehydrogenase as a Universal Biosensor Platform
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DOI：
10.1021/jacs.6b06342
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发表时间：
2016-08-17
影响因子：
15
通讯作者：
Alexandrov, Kirill
中科院分区：
文献类型：
--
作者：
Guo, Zhong;Murphy, Lindy;Alexandrov, Kirill
具有直接电子输出的生物传感器有望与便携式电子设备几乎无缝集成。然而，到目前为止，它们都是基于天然存在的酶，这些酶显著限制了可检测分析物的光谱。在这里，我们提出了一种新的生物传感器架构的基础上分析物驱动的分子间重组和活性重建的重组组件的血糖仪：PQQ-葡萄糖脱氢酶。我们证明，这种传感器架构可以快速通过检测免疫抑制药物，α-淀粉酶蛋白，或凝血酶和因子Xa的蛋白酶活性。生物传感器可以以干燥的形式储存而没有明显的活性损失。我们进一步表明，配体诱导的活性的开发的生物传感器可以直接监测计时电流，使一次性传感电极的建设。我们希望这种架构可以扩展到其他生化活动，翻译后修饰，核酸和无机分子的检测。
Biosensors with direct electron output hold promise for nearly seamless integration with portable electronic devices. However, so far, they have been based on naturally occurring enzymes that significantly limit the Spectrum of detectable analytes. Here, we present a novel biosensor architecture based on analyte-driven intermolecular recombination and activity reconstitution of a re engineered component of glucometers: PQQ-glucose dehydrogenase. We demonstrate that this sensor architecture can be rapidly adopted for the detection of immunosuppressant drugs, alpha-amylase protein, or protease activity of thrombin and Factor Xa. The biosensors could be stored in dried form without appreciable loss of activity. We further show that ligand-induced activity of the developed biosensors could be directly monitored by chronoamperoinetry, enabling construction of disposable sensory electrodes. We expect that this architecture could be expanded to the detection of other biochemical activities, post-translational modifications, nucleic acids, and inorganic molecules.