Break-induced replication occurs by conservative DNA synthesis
Break-induced replication occurs by conservative DNA synthesis
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DOI：
10.1073/pnas.1309800110
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发表时间：
2013-08-13
影响因子：
11.1
通讯作者：
Symington, Lorraine S.
中科院分区：
文献类型：
--
作者：
Donnianni, Roberto A.;Symington, Lorraine S.
断裂诱导的复制（BIR）是指从DNA双链断裂的一端开始并可延伸超过100 kb的重组依赖性DNA合成。BIR由Rad 51催化的链侵入启动，但DNA合成的机制尚不清楚。在这里，我们使用BrdU掺入跟踪DNA合成过程中BIR，发现新合成的链分离与断裂的染色体，DNA合成的保守模式的指示。此外，我们表明，BIR的频率降低和产品的形成是逐步延迟时，捐助者被放置在一个越来越大的距离从端粒，与复制由一个迁移的D-环从起始位点的端粒。
Break-induced replication (BIR) refers to recombination-dependent DNA synthesis initiated from one end of a DNA double-strand break and can extend for more than 100 kb. BIR initiates by Rad51-catalyzed strand invasion, but the mechanism for DNA synthesis is not known. Here, we used BrdU incorporation to track DNA synthesis during BIR and found that the newly synthesized strands segregate with the broken chromosome, indicative of a conservative mode of DNA synthesis. Furthermore, we show the frequency of BIR is reduced and product formation is progressively delayed when the donor is placed at an increasing distance from the telomere, consistent with replication by a migrating D-loop from the site of initiation to the telomere.