Novel current-conducting composite substrates for exposing osteoblasts to alternating current stimulation
Novel current-conducting composite substrates for exposing osteoblasts to alternating current stimulation
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DOI：
10.1002/jbm.10015
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发表时间：
2002-03-05
期刊：
影响因子：
--
通讯作者：
Bizios, R
中科院分区：
文献类型：
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作者：
Supronowicz, PR;Ajayan, PM;Bizios, R
目前的研究表明，由聚乳酸和碳纳米管混合物组成的新型纳米复合材料可以有效地用于将细胞暴露在电刺激下。在这些纳米复合材料表面培养的成骨细胞在不同时间的电刺激(10MUA，10 Hz)6h/d，2d后细胞增殖率增加46%，21d后细胞外钙浓度增加307%，连续1d和21d后I型胶原基因表达上调。这些结果证明，通过新型导电聚合物/纳米相复合材料提供的电刺激促进了成骨细胞的功能，而成骨细胞功能负责骨的有机和无机相的化学组成。此外，这一证据阐明了电刺激下新骨形成所涉及的细胞/分子水平的机制。(C)2001 John Wiley&Sons，Inc.生物医学杂志59：499-506,2002。
The present study demonstrates that novel nanocomposites consisting of blends of polylactic acid and carbon nanotubes effectively can be used to expose cells to electrical stimulation. When osteoblasts cultured on the surfaces of these nanocomposites were exposed to electric stimulation (10 muA at 10 Hz) for 6 h/day for various periods of time, there was a 46% increase in cell proliferation after 2 days, a 307% increase in the concentration of extracellular calcium after 21 consecutive days, and upregulation of mRNA expression for collagen type-I after both 1 and 21 consecutive days. These results provide evidence that electrical stimulation delivered through novel, current-conducting polymer/nanophase composites promotes osteoblast functions that are responsible for the chemical composition of the organic and inorganic phases of bone. Furthermore, this evidence elucidates aspects of the cellular/molecular-level mechanisms involved in new bone formation under electrical stimulation. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 499-506, 2002.