Differential expression of two distinct MyoD genes in Xenopus.
Differential expression of two distinct MyoD genes in Xenopus.
复制标题
爪蟾中两个不同 MyoD 基因的差异表达。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
M. Perry
中科院分区：
文献类型：
--
作者：
J. B. Scales;E. Olson;M. Perry
We previously reported the isolation of several complementary DNAs from Xenopus laevis that encode distinct MyoD proteins. Two of these genes, Xlmf1 and Xlmf25, appear to represent a gene duplication as a consequence of the polyploid Xenopus genome. Although both MyoD genes are expressed exclusively in skeletal muscle in adult animals, they have very different temporal patterns of expression in early development. In the present work, we show that Xlmf1 transcripts rapidly accumulated to high levels shortly after activation of the zygotic genome at the midblastula transition. In contrast, Xlmf25 was expressed as a maternal transcript that was maintained at a relatively constant level throughout early development. Xlmf25, like Xlmf1, was capable of converting 10T1/2 fibroblasts to a myogenic phenotype. In addition, both proteins directly transactivated reporter genes linked to muscle-specific regulatory elements. Xlmf1 was twice as active in this regard as Xlmf25 and required a carboxy-terminal domain for its function. The absence of apparent effect of the maternally expressed myogenic gene in early embryos, but not in transfected fibroblasts, suggests the existence of regulatory mechanisms that repress the function of this gene in cells with nonmuscle fates during early amphibian development.
影响因子：
5.3
作者：
GOSSETT, LA;KELVIN, DJ;OLSON, EN
通讯作者：
OLSON, EN