Characterization and analysis of an industrial strain of Streptomyces bingchenggensis by genome sequencing and gene microarray.
Characterization and analysis of an industrial strain of Streptomyces bingchenggensis by genome sequencing and gene microarray.
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DOI：
10.1139/gen-2013-0098
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发表时间：
2013-11
期刊：
影响因子：
3.1
通讯作者：
Xiangjing Wang;Bo Zhang;Yi-Jun Yan;Jing An;Ji Zhang;Chongxi Liu;W. Xiang
中科院分区：
文献类型：
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作者：
Xiangjing Wang;Bo Zhang;Yi-Jun Yan;Jing An;Ji Zhang;Chongxi Liu;W. Xiang
宾城链霉菌是一种土壤细菌，产生米尔贝霉素，米尔贝霉素是一种大环内酯类抗生素家族，在作物保护和兽医学中具有重要的商业意义。此外，S.此外，该属植物还产生聚醚南昌霉素和新型环五肽等多种天然产物。为了确定参与这些化合物生物合成的基因簇，并更好地阐明这些基因簇的生化途径，对S。测序结果表明，该基因的转录组表达谱与其表达量呈正相关。与其它链霉菌基因组序列比较，S.冰城种具有最大的线性染色体，由11936683bp组成，平均GC含量为70.8%。10023个预测的蛋白质编码序列包括至少47个与已知或预测的次级代谢产物的生物合成相关的基因簇。转录分析表明，在整个生长期的milbemycin基因簇的表达水平极高，南昌霉素基因簇的表达水平在最初的几个小时，随后下降的中等高。然而，其他基因簇似乎是沉默的。利用全基因组分析方法，对S.冰城菌株的研究，加上转录分析，将促进米尔贝霉素高产菌株的合理开发以及新天然产物的发现。
Streptomyces bingchenggensis is a soil bacterium that produces milbemycins, a family of macrolide antibiotics that are commercially important in crop protection and veterinary medicine. In addition, S. bingchenggensis produces many other natural products including the polyether nanchangmycin and novel cyclic pentapeptides. To identify the gene clusters involved in the biosynthesis of these compounds, and better clarify the biochemical pathways of these gene clusters, the whole genome of S. bingchenggensis was sequenced, and the transcriptome profile was subsequently investigated by microarray. In comparison with other sequenced genomes in Streptomyces, S. bingchenggensis has the largest linear chromosome consisting of 11 936 683 base pairs (bp), with an average GC content of 70.8%. The 10 023 predicted protein-coding sequences include at least 47 gene clusters correlated with the biosynthesis of known or predicted secondary metabolites. Transcriptional analysis demonstrated an extremely high expression level of the milbemycin gene cluster during the entire growth period and a moderately high expression level of the nanchangmycin gene cluster during the initial hours that subsequently decreased. However, other gene clusters appear to be silent. The genome-wide analysis of the secondary metabolite gene clusters in S. bingchenggensis, coupled with transcriptional analysis, will facilitate the rational development of high milbemycins-producing strains as well as the discovery of new natural products.