Effect of magnesium-dependent cell membrane alterations on the transport of K+ in Ehrlich ascites tumour cells.
Effect of magnesium-dependent cell membrane alterations on the transport of K+ in Ehrlich ascites tumour cells.
复制标题
镁依赖性细胞膜改变对艾利希腹水肿瘤细胞中 K 转运的影响。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
H. Hoppe
中科院分区：
文献类型：
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作者：
K. Schilling;H. Börnig;G. Cumme;H. Hoppe
肿瘤细胞的镁缺乏或镁负荷会改变细胞膜的通透性。使用 86Rb+ 和 K+ 类似物研究了这种变化对 K+ 跨膜转运的影响。对于池大小不稳定的两室系统，通过数学方法估计流入和流出速率的时间过程。细胞两种状态的比较表明，在缺镁细胞中，被动 K+ 流出显着增强 (40%)。这反过来又刺激 (Na+-K+)-ATP 酶介导的主动反向转运，使 ATP 消耗增加约 30%。然而，在这些条件下，该酶无法维持细胞 K+ 含量。由于最初的流入量增加而出现短暂的短暂增加后，被动净流出占主导地位。细胞两种状态的电泳迁移率的差异证实了细胞膜结构的镁依赖性变化。
Mg-deficiency or Mg-loading of tumour cells changes the permeability of the cell membrane. The influence of this change on the K+ transport across the membrane was investigated using 86Rb+ and K+ analog. The time course of the influx and efflux rates were estimated by means of a mathematical approach for a two-compartment system with inconstant pool sizes. The comparison of the two states of the cells demonstrates that in Mg-deficient cells the passive K+ efflux is significantly enhanced (40%). This in turn stimulates the active counter transport mediated by the (Na+-K+)-ATPase, raising the ATP consumption by about 30%. However, the enzyme is not able to maintain the cellular K+ content under these conditions. After a short transient increase due to the initially enhanced influx the passive net efflux prevails. Differences in the electrophoretic mobility of the two states of the cells confirm Mg-dependent changes of the cell membrane structure.