Morphology and function of blood monocytes after incubation with lung surfactant.
Morphology and function of blood monocytes after incubation with lung surfactant.
复制标题
与肺表面活性剂孵育后血单核细胞的形态和功能。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
B. Robertson
中科院分区：
文献类型：
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作者：
A. Wiernik;T. Curstedt;A. Johansson;C. Jarstrand;B. Robertson
将人血单核细胞与肺表面活性剂（磷脂浓度1- 2.5mg/ml）孵育不同的时间段。在短期（30分钟）孵育后，无论是在静息状态还是在E.大肠杆菌细菌，并增强摄取荧光素标记的酵母颗粒。相同单核细胞的电子显微镜检查显示具有许多突起的活性细胞表面。与表面活性剂长期（24小时）孵育导致细胞粘附在塑料培养皿上的能力降低。虽然静息单核细胞的NBT还原在与表面活性剂长期孵育后增加，但在与细菌刺激后NBT还原的额外增强降低。这些细胞呈圆形，通常缺乏表面结构，细胞核浓缩，细胞质充满表面活性物质。因此，单核细胞最初在表面活性剂存在下被激活，但如果细胞被表面活性剂脂质过度喂养，则其功能能力降低。
Human blood monocytes were incubated for different periods of time with lung surfactant (phospholipid concentration 1-2.5 mg/ml). After short-term (30 min) incubation, there was an increase in the nitroblue tetrazolium (NBT) reduction of the monocytes both at rest and during stimulation with E. coli bacteria, and enhanced ingestion of fluorescein-labelled yeast particles. Electron microscopic examination of the same monocytes showed an active cell surface with numerous protrusions. Long-term (24 h) incubation with surfactant resulted in a reduced ability of the cells to adhere to plastic dishes. Although the NBT-reduction of resting monocytes was increased after long-term incubation with surfactant, the additional enhancement of NBT-reduction after stimulation with bacteria was decreased. These cells were rounded, usually devoid of surface structures, their nuclei were condensed, and their cytoplasm filled with surfactant material. Thus, monocytes are initially activated in the presence of surfactant, but if the cells become overfed with surfactant lipids their functional capacity decreases.