Rapid Algal Culture Diagnostics for Open Ponds Using Multispectral Image Analysis
Rapid Algal Culture Diagnostics for Open Ponds Using Multispectral Image Analysis
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DOI：
10.1002/btpr.1843
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Berberoglu, Halil
中科院分区：
文献类型：
--
作者：
Murphy, Thomas E.;Macon, Keith;Berberoglu, Halil
本文提出了一种多光谱图像分析方法，用于探测藻类培养物的光谱后向散射辐照度。它展示了如何使用这种光谱信息来测量藻类生物量浓度，检测入侵物种，并实时监测养殖健康。为了实现这一目标，使用传统的RGB相机作为三波段光电探测器，对绿藻小球藻的培养物进行成像。和蓝藻变种鱼腥藻。在培养液中放置了一个新型的浮动参比平台，提高了图像颜色强度对生物量浓度的敏感度。在培养图像的RGB颜色向量与每个菌株的单一培养的生物量浓度之间建立了相关性。这些关联式预测了独立培养物的生物量浓度，平均误差分别为22%和14%。此外，利用这两个菌株之间光谱特征的差异来检测小球藻的入侵。变色曲霉培养物。成功地检测到变色拟青霉对小球藻的入侵。质量比小到0.08。最后，提出了一种利用多光谱成像检测栓皮莲热应力的方法。这些方法可以推广到现场应用，为大规模藻类养殖系统的高效运行提供无延迟的过程控制反馈。(C)2013年美国化学工程师协会生物技术研究所。程序，30：233-240,2014
This article presents a multispectral image analysis approach for probing the spectral backscattered irradiance from algal cultures. It was demonstrated how this spectral information can be used to measure algal biomass concentration, detect invasive species, and monitor culture health in real time. To accomplish this, a conventional RGB camera was used as a three band photodetector for imaging cultures of the green alga Chlorella sp. and the cyanobacterium Anabaena variabilis. A novel floating reference platform was placed in the culture, which enhanced the sensitivity of image color intensity to biomass concentration. Correlations were generated between the RGB color vector of culture images and the biomass concentrations for monocultures of each strain. These correlations predicted the biomass concentrations of independently prepared cultures with average errors of 22 and 14%, respectively. Moreover, the difference in spectral signatures between the two strains was exploited to detect the invasion of Chlorella sp. cultures by A. variabilis. Invasion was successfully detected for A. variabilis to Chlorella sp. mass ratios as small as 0.08. Finally, a method was presented for using multispectral imaging to detect thermal stress in A. variabilis. These methods can be extended to field applications to provide delay free process control feedback for efficient operation of large scale algae cultivation systems. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 30:233-240, 2014