Alpha-2,6-sialylation of L-PHA reactive oligosaccharides and expression of N-acetylglucosaminyltransferase V in human diffuse large B cell lymphoma.
Alpha-2,6-sialylation of L-PHA reactive oligosaccharides and expression of N-acetylglucosaminyltransferase V in human diffuse large B cell lymphoma.
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L-PHA 反应性寡糖的 α-2,6-唾液酸化和 N-乙酰氨基葡萄糖转移酶 V 在人弥漫性大 B 细胞淋巴瘤中的表达。
DOI：
10.3892/or.10.6.1759
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发表时间：
2003
期刊：
影响因子：
4.2
通讯作者：
M. Abe
中科院分区：
文献类型：
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作者：
Osamu Suzuki;Y. Nozawa;T. Kawaguchi;M. Abe
L-PHA 反应性寡糖的细胞表面唾液酸化和 beta1-6 分支在各种肿瘤细胞系的转移能力中发挥着重要作用。我们分析了人弥漫性大 B 细胞淋巴瘤 (DLBCL) 中 L-PHA 反应性寡糖的表达和唾液酸化。 DLBCL 分为三种类型；我）。 A组，非反应型，不表达L-PHA反应性寡糖，ii)。 B组，唾液酸化类型，表达唾液酸化L-PHA反应性寡糖和iii)。 C组，非唾液酸化类型，表达非唾液酸化L-PHA反应性寡糖。为了阐明 B 组病例 L-PHA 反应性寡糖中唾液酸残基的连接，在用两种不同的神经氨酸酶处理后进行了 L-PHA 凝集素组织化学分析。在所有 B 组病例中，用霍乱弧菌神经氨酸酶处理后发现 L-PHA 结合反应性。但用新城疫病毒神经氨酸酶治疗后则不然。这些数据表明，α2,6连接的唾液酸残基主要参与B组L-PHA反应性寡糖的唾液酸化。为了阐明催化L-PHA反应性寡糖的β1-6分支的N-乙酰氨基葡萄糖转移酶V（GnT-V）的表达与DLBCL中的L-PHA反应性之间的关系，我们使用免疫组织化学方法研究了GnT-V的表达。大多数B组和C组病例表达GnT-V，而33%的A组病例不表达GnT-V。这些数据表明 GnT-V 的表达并不总是与 L-PHA 反应性糖缀合物的表达相关。此外，不表达GnT-V的A组患者的生存期明显短于表达GnT-V的C组患者。因此，由于淋巴瘤细胞中缺乏 GnT-V 表达而导致非唾液酸化 L-PHA 反应性寡糖的损失可能与 DLBCL 的侵袭性有关。
Cell surface sialylation and beta1-6 branching of L-PHA reactive oligosaccharides play an important role in metastatic capacities of various tumor cell lines. We analyzed the expression and sialylation of L-PHA reactive oligosaccharides in human diffuse large B cell lymphoma (DLBCL). DLBCL was grouped into three types; i). Group A, non-reactive type with no expression of L-PHA reactive oligosaccharides, ii). Group B, sialylated type with expression of sialylated L-PHA reactive oligosaccharides and iii). Group C, non-sialylated type with expression of non-sialylated L-PHA reactive oligosaccharides. To clarify the linkage of sialic acid residues in L-PHA reactive oligosaccharides of Group B cases, L-PHA lectin histochemistry after treatment with two different neuraminidases was performed. In all Group B cases, L-PHA binding reactivity was found after treatment with Vibrio cholerae neuraminidase. But not after treatment with Newcastle disease virus neuraminidase. These data indicate that alpha2,6-linked sialic acid residues were predominantly involved in sialylation of L-PHA reactive oligosaccharides of Group B. To clarify the relationship between expression of N-acetylglucosaminyltransferase V (GnT-V), which catalyzes beta1-6 branching of L-PHA reactive oligosaccharides, and L-PHA reactivities in DLBCL, we investigated the expression of GnT-V using immunohistochemical methods. Most of the Group B and C cases expressed GnT-V while 33% of Group A cases showed no expression of GnT-V. These data suggest that expression of GnT-V is not always correlated with the expression of L-PHA reactive glycoconjugates. Furthermore, survival of patients in Group A which showed no expression of GnT-V was significantly shorter than that of patients in Group C which expressed GnT-V. Therefore, loss of non-sialylated L-PHA reactive oligosaccharides due to lack of expression of GnT-V in lymphoma cells may be associated with aggressiveness of DLBCL.