A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila
A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila
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DOI：
10.1016/j.mod.2006.07.010
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发表时间：
2006-11-01
影响因子：
2.6
通讯作者：
Sanchez-Herrero, Ernesto
中科院分区：
文献类型：
--
作者：
de Navas, Luis;Foronda, David;Sanchez-Herrero, Ernesto
一种基因产物被另一种基因产物的功能替代是研究发育和进化中特异性的一种强有力的方法。在果蝇中，Ga 14/UAS方法已被用于分析体内这种功能取代。为此目的，需要能够复制a基因并再现其表达模式的Ga 14系，并且它们通常可以通过替换具有这种特征的预先存在的P-lacZ系来获得。我们设计了一种新的方法来快速鉴定P-lacZ系被P-Ga 14系替换，并成功地将其应用于获得Ultrabithorax和Abdominal-B Hox基因中的Ga 14插入。我们用这些细胞系来研究Hox基因被另一个基因的功能替代。我们的实验证实，abdominal-A基因可以取代Ultrabithorax在笼头发育，但它不能取代Abdominal-B在生殖器的形成。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
The functional replacement of one gene product by another one is a powerful method to study specificity in development and evolution. In Drosophila, the Ga14/UAS method has been used to analyze in vivo such functional substitutions. To this aim, Ga14 lines that inactivate a gene and reproduce its expression pattern are required, and they can be frequently obtained by replacing pre-existing P-lacZ lines with such characteristics. We have devised a new method to quickly identify replacements of P-lacZ lines by P-Ga14 lines, and applied it successfully to obtain Ga14 insertions in the Ultrabithorax and Abdominal-B Hox genes. We have used these lines to study the functional replacement of a Hox gene by another one. Our experiments confirm that the abdominal-A gene can replace Ultrabithorax in haltere development but that it cannot substitute for Abdominal-B in the formation of the genitalia. (c) 2006 Elsevier Ireland Ltd. All rights reserved.