A centrifugal microfluidic platform for point-of-care diagnostic applications
A centrifugal microfluidic platform for point-of-care diagnostic applications
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DOI：
10.1590/sajs.2014/20130091
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发表时间：
2014-01-01
影响因子：
2.4
通讯作者：
Kido, Horacio
中科院分区：
文献类型：
--
作者：
Hugo, Suzanne;Land, Kevin;Kido, Horacio
微流体系统能够以紧凑且低成本的形式精确控制微小体积的流体，从而为开发即时诊断解决方案提供了理想的平台。离心微流控系统，也称为盘上实验室或 CD 上实验室系统，由于其简单紧凑的仪器以及功能多样性，为微流护理点诊断解决方案的实施提供了特别有吸引力的解决方案。在这里，我们详细介绍了离心微流体平台的实施——南非首个此类平台——作为开发现场诊断应用的基础，该应用的需求和影响都很大。离心微流控平台由三个主要组件组成：尺寸和形状与CD相似的微流控盘装置、用于控制装置上流体流动的系统以及用于记录所获得结果的系统。这些组件已成功实施和测试。初步测试结果表明，可以成功实现流体泵送和阀门控制等微流体功能，以及单分散微流体液滴的生成，提供完整的离心微流体平台和开发各种应用（包括现场诊断）的构建模块。光盘实验室平台有潜力根据健康和工业相关领域的需求提供新的诊断解决方案。这为向资源有限的地区提供服务铺平了道路，例如改善、分散的医疗保健服务或水质监测，以及以低成本减少诊断时间。
Microfluidic systems enable precise control over tiny volumes of fluid in a compact and low-cost form, thus providing the ideal platform on which to develop point-of-care diagnostic solutions. Centrifugal microfluidic systems, also referred to as lab-on-a-disc or lab-on-a-CD systems, provide a particularly attractive solution for the implementation of microfluidic point-of-care diagnostic solutions as a result of their simple and compact instrumentation, as well as their functional diversity. Here we detail the implementation of a centrifugal microfluidic platform - the first of its kind in South Africa - as a foundation for the development of point-of-care diagnostic applications for which both the need and impact is great. The centrifugal microfluidic platform consists of three main components: a microfluidic disc device similar in size and shape to a CD, a system for controlling fluid flow on the device, and a system for recording the results obtained. These components have been successfully implemented and tested. Preliminary test results show that microfluidic functions such as pumping and valving of fluids can be successfully achieved, as well as the generation of monodisperse microfluidic droplets, providing a complete centrifugal microfluidic platform and the building blocks on which to develop a variety of applications, including point-of-care diagnostics. The lab-on-a-disc platform has the potential to provide new diagnostic solutions at the point-of-need in health-and industry-related areas. This paves the way for providing resource limited areas with services such as improved, decentralised health-care access or water-quality monitoring, and reduced diagnosis times at a low cost.