Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children
Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children
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DOI：
10.1016/j.virol.2013.09.002
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发表时间：
2013-12-01
期刊：
影响因子：
3.7
通讯作者：
Victoria Preciado, Maria
中科院分区：
文献类型：
--
作者：
Ines Gismondi, Maria;Diaz Carrasco, Juan Maria;Victoria Preciado, Maria
采用经典的系统发育和现代基于人群的聚类方法分析了6名儿童在3.2-9.6年的随访期间血浆中丙型肝炎病毒（HCV）的进化，并评估了外周血单个核细胞（PBMCs）中的病毒区隔化。通过对包含高变区1的克隆扩增子的群体结构分析，可以区分出两种进化模式，一种是高度分化的，另一种是遗传同质的。病毒的适应性反映在病毒群落的共同进化中，在高度同质群落的分化和稳定背景下，病毒群落从一个快速切换到另一个，而高度同质群落在很长一段时间后被新的群落所取代。此外，在统计上证实了pbmc中HCV的病毒区隔化，表明它们是遗传变异性的一个库。我们的研究结果支持慢性感染期间HCV病毒种群的社区结构的观点，并强调了PBMC室在这种结构的持久性中的作用。(C) 2013爱思唯尔公司版权所有。
Classic phylogenetic and modern population-based clustering methods were used to analyze hepatitis C virus (HCV) evolution in plasma and to assess viral compartmentalization within peripheral blood mononuclear cells (PBMCs) in 6 children during 3.2-9.6 yr of follow-up. Population structure analysis of cloned amplicons encompassing hypervariable region 1 led to the distinction of two evolutionary patterns, one highly divergent and another one genetically homogeneous. Viral adaptability was reflected by co-evolution of viral communities switching rapidly from one to another in the context of divergence and stability associated with highly homogeneous communities which were replaced by new ones after long periods. Additionally, viral compartmentalization of HCV in PBMCs was statistically demonstrated, suggesting their role as a pool of genetic variability.Our results support the idea of a community-based structure of HCV viral populations during chronic infection and highlight a role of the PBMC compartment in the persistence of such structure. (C) 2013 Elsevier Inc. All rights reserved.