Effect of Different levels of NaCl Salinity on Antioxidant Enzyme's Activity in Seedling of Different Wheat Cultivars

Effect of Different levels of NaCl Salinity on Antioxidant Enzyme's Activity in Seedling of Different Wheat Cultivars
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发表时间:
2015
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通讯作者:
H. Nabizadeh;M. Valizadeh;M. Norouzi;M. Toorchi;Mohammad Behrouzi Vajovi
H. Nabizadeh;M. Valizadeh;M. Norouzi;M. Toorchi;Mohammad Behrouzi Vajovi
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作者:
H. Nabizadeh;M. Valizadeh;M. Norouzi;M. Toorchi;Mohammad Behrouzi Vajovi

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小麦是谷物中最重要的,也是全球最古老的作物之一,因此根据伊朗的气候条件,可以得出结论,对该作物产生不利影响的最关键/限制性胁迫之一是盐胁迫。因此,在盐胁迫下,在分子水平上对小麦进行基因型评估是一个强制性目标。为此,研究了14个小麦品种苗期过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POX)等酶在对照(0)、弱胁迫(100 mM)中度胁迫(200 mM)和重度胁迫(300 mM)4种盐度处理下的活性。酶活性的定量达达方差分析表明盐胁迫对某些POX同工酶的活性有显着影响。另一方面,在不同的小麦品种中,除 CAT 外,所有检测到的同工酶活性均表现出显着差异。
Wheat is of paramount importance among cereals and one of the most ancient crops in the globe, so according to Iran's climate condition, it could be concluded that one of the most crucial/limiting stresses which adversely affects this crop is salt stress. Therefore, genotypic evaluation of wheat at a molecular level, while under salt stress, is a mandatory objective. In this regard, the activity of enzymes including catalase (CAT), superoxide dismutase (SOD), and peroxidase (POX) of 14 wheat cultivars during seedling stage was investigated under four salinity treatments, the control (0),weak stress(100mM) moderate stress (200mM), and severe stress (300mM). Quantified dada variance analysis of enzymes activity showed that salt stress has significant effect on activity of some POX isozymes. On the other extreme, among different cultivars of wheat the activity of all detected isoenzymes showed significant differences with the exception of CAT.