Simple colorimetric inhibition assay of heme crystallization for high-throughput screening of antimalarial compounds
Simple colorimetric inhibition assay of heme crystallization for high-throughput screening of antimalarial compounds
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DOI：
10.1128/aac.00985-06
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Kamei, Kaeko
中科院分区：
文献类型：
--
作者：
Huy, Nguyen Tien;Uyen, Dinh Thanh;Kamei, Kaeko
目前用于筛选新抗疟药的测定需要β-正铁血红素形成的引发剂，这需要费力的制备、特殊的装置和底物。在这项研究中，基于减少血红素吸收的β-高铁血红素形成，我们开发了一个简单的比色法使用吐温20作为引发剂和酶标仪的高通量筛选抑制剂的β-高铁血红素形成。
Current assays for screening new antimalarials need initiators of beta-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction of heme absorption in beta-hematin formation, we developed a simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of beta-hematin formation.