Vascular washout reduces Ca2+ overload and improves function of reperfused ischemic hearts.
Vascular washout reduces Ca2+ overload and improves function of reperfused ischemic hearts.
复制标题
血管冲洗可减少 Ca2+ 超载并改善再灌注缺血心脏的功能。
DOI：
10.1152/ajpheart.1990.258.2.h354
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Neely,JR
中科院分区：
文献类型：
--
作者：
Tani,M;Neely,JR
心肌钙摄取，心室功能的恢复，并恢复组织代谢产物之间的关系进行了测定，在30分钟的缺血和缺氧灌注后，无论是零或低冠状动脉流量，零流量间歇灌注，低流量灌注无底物。当零流量缺血维持30或40分钟，组织乳酸水平增加约100倍，与这些心脏的再灌注，开发的压力恢复到只有70和40%的缺血前功能，分别和Ca 2+摄取增加了7和15倍。相比之下，30分钟的低流量（1毫升/分钟）缺氧灌注导致积累较少的乳酸（15倍增加），再灌注Ca 2+的吸收，并恢复开发的压力缺氧前的水平。在低流量缺氧灌注过程中省略能量底物导致心率恢复减少，高能磷酸盐水平降低，Ca 2+摄取增加，但收缩功能恢复到与低流量灌注底物相同的程度。即使是非常低的流量（0.06-0.16 ml/min）的缺氧灌注液增加高能磷酸盐含量和收缩功能，并减少Ca 2+摄取。在4次10分钟的缺血发作之间间歇灌注含氧或缺氧缓冲液，可减少乳酸盐积累，维持功能，并使Ca 2+摄取基本不变。恢复开发的压力在再灌注过程中呈负相关，与缺血或缺氧过程中积累的乳酸盐的量，并与再灌注Ca 2+摄取，无论缺血或缺氧的持续时间或类型。（250字处删节）
Relationships between myocardial Ca2+ uptake, recovery of ventricular function, and restoration of tissue metabolites were determined during 30 min of reperfusion following ischemic and anoxic perfusion with either zero or low coronary flow, zero flow with intermittent perfusion, and low-flow perfusion without substrates. When zero-flow ischemia was maintained for 30 or 40 min, tissue lactate levels increased approximately 100-fold; with reperfusion of these hearts, developed pressure recovered to only 70 and 40% of preischemic function, respectively, and Ca2+ uptake increased by 7- and 15-fold. In contrast, 30 min of low-flow (1 ml/min) anoxic perfusion resulted in accumulation of less lactate (15-fold increase), less reperfusion Ca2+ uptake, and recovery of developed pressure to the preanoxic level. Omission of energy substrates during the low-flow anoxic perfusion caused a reduced recovery of heart rate with lower high-energy phosphate levels and increased Ca2+ uptake, but contractile function recovered to the same extent as in low-flow perfusion with substrate. Even very low flow rates (0.06-0.16 ml/min) of oxygen-deficient perfusate increased high-energy phosphate content and contractile function and decreased Ca2+ uptake. Intermittent perfusion with either oxygenated or anoxic buffer between four 10-min episodes of ischemia reduced lactate accumulation, maintained function, and left Ca2+ uptake essentially unchanged. Recovery of developed pressure during reperfusion was negatively correlated with the amount of lactate that accumulated during ischemia or anoxia and with reperfusion Ca2+ uptake, regardless of the duration or type of ischemia or anoxia.(ABSTRACT TRUNCATED AT 250 WORDS)