Let-7b and microRNA-199a inhibit the proliferation of B16F10 melanoma cells
Let-7b and microRNA-199a inhibit the proliferation of B16F10 melanoma cells
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Let-7b和microRNA-199a抑制B16F10黑色素瘤细胞的增殖
DOI：
10.3892/ol.2012.878
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发表时间：
2012-11-01
期刊：
影响因子：
2.9
通讯作者：
Zhou, Jianda
中科院分区：
文献类型：
--
作者：
Xu, Dan;Tan, Jianxiang;Zhou, Jianda
皮肤黑色素瘤是人类皮肤癌的一种侵袭性形式，其特征在于高转移潜能和不良预后。迫切需要转移风险的生物标志物，以在高危患者中采取新的辅助措施。在皮肤黑色素瘤的临床标本中，我们先前发现let-7 b、microRNA-199 a和microRNA-33与转移性黑色素瘤显著相关，因此可能是黑色素瘤治疗的关键。在这项研究中，我们检查了let-7 b和microRNA-199 a的过表达和抑制的效果。将过表达这些基因的质粒转染入B16 F10黑色素瘤细胞中，并在RNA、蛋白质和细胞水平上评估let-7 b和microRNA-199 a的表达。转染let-7 b质粒和let-7 b抑制剂的细胞中Cyclin D1的表达明显高于对照组（P
Cutaneous melanoma is an aggressive form of human skin cancer characterized by high metastatic potential and poor prognosis. Biomarkers of metastatic risk are critically needed to instigate new auxiliary measures in high-risk patients. In clinical specimens of skin melanoma, we previously found that let-7b, microRNA-199a and microRNA-33 were significantly associated with metastatic melanoma, and thus may be the key to melanoma treatment. In this study, we examined the effect of overexpression and inhibition of let-7b and microRNA-199a. Plasmids overexpressing these genes were transfected into B16F10 melanoma cells, and let-7b and microRNA-199a expression were evaluated at the RNA, protein and cellular level. Cyclin D1 expression was significantly higher in cells transfected with let-7b plasmid and let-7b inhibitor compared with control cells (P