The sympathetic-vagal balance against endotoxemia
The sympathetic-vagal balance against endotoxemia
复制标题
DOI：
10.1007/s00702-010-0407-6
复制
发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Huang, Xiankai
中科院分区：
文献类型：
--
作者：
Huang, Jian;Wang, Yaoli;Huang, Xiankai
本研究的目的是通过迷走神经刺激（VNS）重新平衡交感-迷走神经系统来建立一种方便有效的抗炎治疗方法。采用脂多糖（LPS）注射法建立SD大鼠内毒素血症模型。放电的迷走神经系统，包括孤束核（NTS）和传入和传出颈迷走神经，进行了检测。交感神经迷走神经平衡的条件，表示为心率变异性（HRV）和肝去甲肾上腺素/乙酰胆碱（NE/ACh），测量后内毒素血症与VNS和不。与对照组的基础水平和相应时间点相比，注射LPS后迷走神经传入和传出的放电均显著增加。LPS注射后，NTS的放电也显著增加。注射LPS后，HRV各成分（包括标准化高频（HFnm）、标准化低频（LFnm）、LF/HF和极低频（VLF））均显著增加。与LPS组相比，LPS + VNS组HFnm值明显增加。而LPS + VNS组的LFnm、LF/HF、VLF较LPS组明显降低。注射LPS后6 h内，肝脏NE和ACh均较基础水平和对照组显著降低（P < 0.05）。VNS对肝NE无明显改善作用，但LPS + VNS组ACh水平明显高于其他各组。内毒素血症后交感神经和迷走神经系统增强。交感神经系统的过度兴奋导致交感-迷走神经失衡。交感神经和迷走神经系统的再平衡对于重症患者至关重要。
The goal of this study was to establish a convenient and effective approach to anti-inflammation treatment by rebalancing the sympathetic-vagal system via vagal nerve stimulation (VNS). We established an endotoxemia model in Sprague-Dawley rats using lipopolysaccharide (LPS) injection. Electrical discharges in the vagal system, including the nucleus tractus solitarii (NTS) and afferent and efferent cervical vagal nerves, were detected. The condition of sympathetic-vagal balance, presented as heart rate variability (HRV) and hepatic norepinephrine/acetylcholine (NE/ACh), was measured following endotoxemia with and without VNS. Discharges in afferent and efferent vagal nerves increased significantly following LPS injection compared with the basis level and corresponding time points in the control group. Discharges in the NTS also increased significantly following LPS injection. The HRV components, including normalized high frequency (HFnm), normalized low frequency (LFnm), LF/HF, and very low frequency (VLF), increased significantly following LPS injection. HFnm values in the LPS + VNS group increased significantly compared with the LPS group. Conversely, LFnm, LF/HF, and VLF in the LPS + VNS group decreased significantly compared with the LPS group. Hepatic NE and ACh significantly decreased within 6 h after LPS injection compared with the basal level and the control groups (P < 0.05). VNS did not significantly improve hepatic NE, but the ACh levels in the LPS + VNS group were higher than those in other groups. Sympathetic and vagal nervous systems are enhanced following endotoxemia. The overexcitation of the sympathetic system leads to sympathetic-vagal disequilibrium. The rebalance of the sympathetic and vagal system is crucial for critically ill patients.