Expression and localization of fibroblast growth factor (FGF) family members during the final growth of bovine ovarian follicles
Expression and localization of fibroblast growth factor (FGF) family members during the final growth of bovine ovarian follicles
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DOI：
10.1002/mrd.10386
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发表时间：
2004-02-01
影响因子：
2.5
通讯作者：
Schams, D
中科院分区：
文献类型：
--
作者：
Berisha, B;Sinowatz, F;Schams, D
本研究的目的是研究成纤维细胞生长因子（FGF）家族成员：FGF 1，FGF 2和FGF 7，及其受体变体：FGFR：FGFR 2 IIIb和FGFR 2 IIIc在牛卵泡内膜（TI）和颗粒细胞（GC）在最终生长过程中的可能参与。根据卵泡液（FF）雌二醇-17 β（E）含量将卵泡分为五组（分别为0.5-5; >5-20; >20-180; >180 ng/ml）。采用逆转录-聚合酶链反应（RT-PCR）和免疫组织化学方法分析mRNA表达和蛋白定位。FGF 1 mRNA表达在TI中相对较高，在GC中较低，并且在最终卵泡生长期间两个组织隔室没有任何调节变化。FGF 1蛋白主要定位于GC的细胞质中，血管平滑肌细胞中，卵巢网中，并且在较小程度上在卵泡膜细胞中。在大卵泡中，TI中的FGF 2 mRNA显著增加，而在GC中，TI中的FGF 2 mRNA较低，且无任何调节变化。FGF 7 mRNA在TI中表达相对较高，而在GC中表达极低。对于成熟卵泡中的FGF 7，可以证明TI和GC基底层的显著染色。TI中FGFR的mRNA信号随着E产生的开始而显著增加（E > 0.5-5 ng/ml FF），并且在GC中没有任何调节变化。FGFR 2 IIIb mRNA在GC中的表达相对较高，并且在卵泡的最终生长过程中显著增加，而TI的表达非常低。TI和GC中FGFR 2 IIIc mRNA表达相对较高，但无明显变化。我们的研究结果表明，FGF生长因子家族成员参与卵泡发生的过程中，特别是在排卵前（占主导地位）的卵泡通过刺激血管生成和GC的生存和增殖的最终增长。(C)2004 Wiley-Liss，Inc.
The aim of this study was to investigate the possible participation of fibroblast growth factor (FGF) family members: FGF1, FGF2 and FGF7, and their receptor variants: FGFR: FGFR2IIIb, and FGFR2IIIc in theca interna (TI) and granulosa cell (GC) compartments of bovine follicles during final growth. A classification of follicles into five groups (0.5-5; >5-20; >20-180; >180 ng/ml, respectively) was performed according to the follicular fluid (FF) oestradiol-17beta (E) content. The mRNA expression and protein localization was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. FGF1 mRNA expression was relatively high in TI and lower in GC, and without any regulatory change for both tissue compartments during final follicular growth. The FGF1 protein could be predominantly localized in the cytoplasm of GC, in smooth muscle cells of blood vessels, in the rete ovarii, and at a lesser degree in theca cells. FGF2 mRNA in TI increased significantly in large follicles and was low and without any regulatory change in GC. FGF7 mRNA expression was relatively high in TI and very low in GC. For FGF7 in mature follicles a marked staining of the TI and the basal layers of the GC could be demonstrated. The mRNA signal for the FGFR in TI increased significantly with beginning of E production (E > 0.5-5 ng/ml FF) and was without any regulatory change in GC. The mRNA expression of FGFR2IIIb was relatively high in GC and increased significantly during final growth of follicles in contrast to the TI with very low expression. The FGFR2IIIc mRNA expression in TI and GC was relatively high but without any clear change. Our results suggest that FGF growth factor family members are involved in process of folliculogenesis and especially during final growth of the preovulatory (dominant) follicle by stimulation of angiogenesis and GC survival and proliferation. (C) 2004 Wiley-Liss, Inc.