Development of a highly permissive cell line from spotted knifejaw (Oplegnathus punctatus) for red sea bream iridovirus
Development of a highly permissive cell line from spotted knifejaw (Oplegnathus punctatus) for red sea bream iridovirus
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DOI：
10.1016/j.aquaculture.2017.02.027
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发表时间：
2017-04-20
期刊：
影响因子：
4.5
通讯作者：
Nakajima, Kazuhiro
中科院分区：
文献类型：
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作者：
Kawato, Yasuhiko;Yamashita, Hirofumi;Nakajima, Kazuhiro
我们从斑点刀颌鱼 (Oplegnathus punctatus) 中开发了一种细胞系 (SKF-9)，该细胞系对红鲷虹彩病毒 (RSIV) 高度敏感。在 RSIV 感染的 SKF-9 细胞中清楚地观察到以变圆和增大为特征的细胞病变效应 (CPE)。 SKF-9 细胞的病毒产量大约比传统用于 RSIV 研究的 GF 细胞高 100 倍。使用 SKF-9 细胞传代时，病毒滴度并未降低，而使用 GF 细胞时，观察到滴度显着降低。另一方面，控制适当的通道数（
We developed a cell line (SKF-9) from spotted knifejaw (Oplegnathus punctatus), which is highly susceptible to red sea bream iridovirus (RSIV). Cytopathic effect (CPE) characterized by rounding and enlargement was clearly observed in RSIV-infected SKF-9 cells. The amount of viral production of SKF-9 cells was approximately 100 times greater than that of GF cells, which is conventionally used for RSIV research. The viral titer was not decreased through passages using SKF-9 cells, whereas a considerable reduction in the titer was observed when GF cells were used. On the other hand, control of appropriate passage numbers (